Amy Shen,
Professor and Provost,
Okinawa Institute of Science and Technology Graduate University
Amy Shen is the provost and professor at Okinawa Institute of Science and Technology Graduate University (OIST) in Japan, leading the Micro/Bio/Nanofluidics Unit. Amy's research is focused on microfluidics and lab-on-a-chip devices at the bio/nano-interface, with applications in biotechnology. She is a Fellow of the American Physical Society, the Royal Society of Chemistry, and Society of Rheology. Amy was also a Fulbright Scholar in 2013. She is an associate editor for Soft Matter and serves on the editorial advisory board for ACS Sensors, Journal of Rheology, and Physics of Fluids.
Detection of Antibodies Against SARS-CoV-2 Spike Protein by Gold Nanospikes in an Optomicrofluidic Chip
Wednesday, 9 September 2020 at 09:00
Add to Calendar ▼2020-09-09 09:00:002020-09-09 10:00:00Europe/LondonDetection of Antibodies Against SARS-CoV-2 Spike Protein by Gold Nanospikes in an Optomicrofluidic ChipOrgan-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
The ongoing global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to active research in its associated diagnostics and medical treatments. While quantitative reverse transcription polymerase chain reaction (qRT--PCR) is the most reliable method to detect viral genes of SARS-CoV-2, serological tests for specific antiviral antibodies are also important as they identify false negative qRT--PCR responses, track how effectively the patient's immune system is fighting the infection, and are potentially helpful for plasma transfusion therapies. In this work, based on the principle of localized surface plasmon resonance (LSPR), we develop an optomicrofluidic sensing platform with gold nanospikes, fabricated by electrodeposition, to detect the presence and amount of antibodies specific to the SARS-CoV-2 spike protein in 1 uL of human plasma diluted in 1 mL of buffer solution, within ~30~min. The target antibody concentration can be correlated with the LSPR wavelength peak shift of gold nanospikes caused by the local refractive index change due to the antigen-antibody binding. This label-free microfluidic platform achieves a limit of detection of ~0.08~ng/mL (~0.5~pM), falling under the clinical relevant concentration range. We demonstrate that our opto-microfluidic platform offers a promising point-of-care testing tool to complement standard serological assays and make SARS-CoV-2 quantitative diagnostics easier, cheaper, and faster.
Add to Calendar ▼2020-09-09 00:00:002020-09-10 00:00:00Europe/LondonOrgan-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020Organ-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2020-09-09 09:00:002020-09-09 10:00:00Europe/LondonDetection of Antibodies Against SARS-CoV-2 Spike Protein by Gold Nanospikes in an Optomicrofluidic ChipOrgan-on-a-Chip, Tissue-on-a-Chip and Organoids Europe 2020 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com
