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SELECTBIO Conferences Organ-on-a-Chip and 3D-Culture: Companies, Technologies and Approaches

Ashutosh Agarwal's Biography

Ashutosh Agarwal, Assistant Professor, Department of Biomedical Engineering, University of Miami Miller School of Medicine

Ashutosh Agarwal, Ph.D., is an Assistant Professor of Biomedical Engineering and Pathology and a core faculty member of the Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute at the University of Miami (BioNIUM). He received his Ph.D. in Materials Science and Engineering at the University of Florida in 2009 and postdoctoral research experience at Columbia University and Harvard University. Dr. Agarwal joined the University of Miami as a faculty member in 2014 where he heads the Physiomimetic Microsystems Laboratory. His research laboratory is focused on developing organ on chip platforms that mimic human organ level complexity within a fluidic microsystem capable of measuring functional readouts.

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Resealable, Optically accessible, PDMS-free Fluidic Platforms for Organs on Chips

Tuesday, 11 July 2017 at 09:00

Add to Calendar ▼2017-07-11 09:00:002017-07-11 10:00:00Europe/LondonResealable, Optically accessible, PDMS-free Fluidic Platforms for Organs on

We report the design and fabrication of a robust fluidic platform built out of inert plastic materials and micro-machined features that promote optimized convective fluid transport. The platform is tested for perfusion interrogation of rodent and human pancreatic islets, dynamic secretion of hormones, concomitant live-cell imaging, and optogenetic stimulation of genetically engineered islets. A coupled quantitative fluid dynamics computational model of glucose stimulated insulin secretion and fluid dynamics was first utilized to design device geometries that are optimal for complete perfusion of three-dimensional islets, effective collection of secreted insulin, and minimization of system volumes and associated delays. Fluidic devices were then fabricated through rapid prototyping techniques, such as micromilling and laser engraving, as two interlocking parts from materials that are non-absorbent and inert. Finally, the assembly was tested for performance using both rodent and human islets with multiple assays conducted in parallel, such as dynamic perfusion, staining and optogenetics on standard microscopes, as well as for integration with commercial perfusion machines. The optimized design of convective fluid flows, use of bio-inert and non-absorbent materials, reversible assembly, manual access for loading and unloading of islets, and straightforward integration with commercial imaging and fluid handling systems proved to be critical for perfusion assay, and particularly suited for time-resolved optogenetics studies.

Add to Calendar ▼2017-07-10 00:00:002017-07-11 00:00:00Europe/LondonOrgan-on-a-Chip and 3D-Culture: Companies, Technologies and