He was trained as an engineer in biotechnologies and specialized in the biomedical field. After his studies, he first worked in a research team at the Institut Cochin to study the effect of the microenvironment on neural stem cell differentiation. He then joined the Alvéole’s team in 2015 in which he is now research scientist in cell biology.
PRIMO: Quantitative Photopatterning of Proteins for Controlling the Cellular Microenvironment
Tuesday, 17 October 2017 at 13:00
Add to Calendar ▼2017-10-17 13:00:002017-10-17 14:00:00Europe/LondonPRIMO: Quantitative Photopatterning of Proteins for Controlling the Cellular MicroenvironmentBioprinting and 3D Printing in the Life Sciences Europe in Cripps Court, Magdalene College, Cambridge, UK Cripps Court, Magdalene College, Cambridge, UK SELECTBIOenquiries@selectbiosciences.com
Cell biology is faced with significant challenges when attempting to create complex microenvironments to mimic the in vitro conditions. These challenges can be overcome by molecular printing on cell culture surfaces, which involves the controlled deposition of proteins on a substrate at micrometer scale to control cell adhesion. This approach has developed tremendously in the past few years, however most micropatterning methods are still limited to only one type of molecule per pattern and do not allow for gradient patterning. We recently developed a new device – PRIMO – allowing for quantitative multi-protein micropatterning. The PRIMO photopatterning technique is based on the technology named “Light-Induced Molecular Adsorption of Proteins (LIMAP)” and uses a water-soluble photo-initiator (PLPP) able to reverse the antifouling property of polymer brushes when exposed to UV light. Our optical setup is composed of a wide-field DMD based projection system coupled to a conventional epifluorescence microscope. It allows to generate any shape of patterns with a resolution down to 1.2 µm, gradients of density of proteins and to control the pattern alignment for multi-protein patterning or for patterning on microstructures. The range of applications of this technology extends from the single molecule up to the multicellular scale with an exquisite control over local protein density. We show that it can be used to generate complex and dynamic protein landscapes useful for cell-cell and cell-matrix interactions studies. Moreover, the DMD-based spatial control of light can be combined with functionalized UV-sensitive hydrogels precursors opening new doors to highly controlled 3D cell cultures.
Add to Calendar ▼2017-10-17 00:00:002017-10-18 00:00:00Europe/LondonBioprinting and 3D Printing in the Life Sciences EuropeBioprinting and 3D Printing in the Life Sciences Europe in Cripps Court, Magdalene College, Cambridge, UK Cripps Court, Magdalene College, Cambridge, UK SELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2017-10-17 13:00:002017-10-17 14:00:00Europe/LondonPRIMO: Quantitative Photopatterning of Proteins for Controlling the Cellular MicroenvironmentBioprinting and 3D Printing in the Life Sciences Europe in Cripps Court, Magdalene College, Cambridge, UK Cripps Court, Magdalene College, Cambridge, UK SELECTBIOenquiries@selectbiosciences.com
