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SELECTBIO Conferences Organ-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions

Muhammad Ibrahim's Biography

Muhammad Ibrahim, Researcher, Institute of Biology, Leiden University

Institute of Biology, Leiden University, the Netherlands.

Muhammad Ibrahim Image

Towards the Growth of Blood Vessel Networks in Microfluidic Culture

Friday, 8 July 2016 at 16:30

Add to Calendar ▼2016-07-08 16:30:002016-07-08 17:30:00Europe/LondonTowards the Growth of Blood Vessel Networks in Microfluidic

One of the major limitations in culturing complex tissues or organs is the lack of vascularization in the cultured tissue. Development of a functional capillary bed in 3D microfluidic culture could overcome this problem. Unlike static culture systems, microfluidic cultures allow the flow of medium to mimic blood flow in vivo, hence allowing the endothelial cells in culture to develop into functional blood vessels with lumina. In this presentation the importance of 3D cell culture and microfluidic technology for development of in vitro vascular network will be discussed.  We are using zebrafish embryonic cells as a low-cost, high-throughput replacement for mammalian cell culture models, currently used for in vitro vasculogenesis and angiogenesis studies. Aggregates of embryonic blastocyst cells (embryoid bodies), containing differentiated endothelial cells, were embedded in a 3D hydrogel and plated into a microfluidic channel. The channel was then connected to a medium reservoir and a microfluidic pump to start the flow of medium through the channel. The results showed significant effects of microfluidic flow on the morphology of blood vessel-like structures formed by the differentiated endothelial cells.

Add to Calendar ▼2016-07-07 00:00:002016-07-08 00:00:00Europe/LondonOrgan-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo