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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics: Companies, Technologies and Commercialization

Abdulghani Ismail's Biography

Abdulghani Ismail, Researcher, Chimie ParisTech, Unité de Technologies Chimiques et Biologiques pour la Santé

Obtained master degree in analytical sciences from Claude Bernard Lyon University (France), in 2013. He worked there on the fabrication of a supernernstian pH meter for integration in microfluidic device in order to differentiate cancerous cells from normal cells. Currently he is a PhD student at “Chimie Paris-Tech, Ecole Nationale Supérieure de Chimie de Paris”, France. His research is centered on the detection of nitric oxide stored under the form of S-nitrosothiols in biological fluid. This detection to be applied in miniaturized devices such as µPADs and other microfluidic devices.

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Analysis of S-Nitrosothiols Using Paper-based Microfluidic Device (µPAD) by Colorimetry: Kinetics of Decomposition and Quantification

Wednesday, 28 September 2016 at 16:00

Add to Calendar ▼2016-09-28 16:00:002016-09-28 17:00:00Europe/LondonAnalysis of S-Nitrosothiols Using Paper-based Microfluidic Device (µPAD) by Colorimetry: Kinetics of Decomposition and

A disposable microfluidic paper-based analytical device (µPAD) was developed to easily analyze different S-nitrosothiols (RSNOs) through colorimetric measurements. RSNOs are carriers of nitric oxide (NO) that plays several physiological and physiopathological roles. The quantitation of RSNOs relies on their decomposition using several protocols and colorimetric detection of the final product, NO or nitrite. µPADs were fabricated by wax printing technology in a circular geometry for decomposition and detection of decayed products interconnected by microfluidic channels and one central sample inlet zone.  Different decomposition protocols including mercuric ion and light (UV, Visible, and Infrared) were tested on µPADs. A 3D printed holder was coupled with µPADs to make easy a simultaneous decomposition procedure using different light sources. Griess reagent was then added to detect NO and nitrite produced by the different decomposition methods. µPADs were then scanned using a flat board scanner. The limit of detection (LOD) values for nitrite and S-nitrosoglutathione (GSNO) using mercuric decomposition were 3 µM and 4 µM, respectively. The LOD reported herein is considered one of the lowest LOD already reported using paper microfluidic devices. The results also show that low molecular weight RSNO, namely S-nitrosocysteine decomposes more easily than high molecular weight RSNOs by light.

Add to Calendar ▼2016-09-26 00:00:002016-09-28 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics: Companies, Technologies and