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SELECTBIO Conferences BioMEMS, Microfluidics & Biofabrication: Technologies and Applications

Yagmur Yildizhan's Biography

Yagmur Yildizhan, Faculty of Engineering and Natural Sciences, Sabanci University Turkey

Yagmur Yildizhan received her B.Sc. degree in Physics Department at Bilkent University in 2016, where she conducted her research related to behavior of microswimmers and drying process of droplets. Currently, she continues her research in Mechatronics Engineering Program at Sabanci University. Her research focuses on dielectrophoretic characterization and separation of cells, particularly M1 and M2 types of macrophages.

Yagmur Yildizhan Image

Characterization and Separation of U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Electrode Dielectrophoresis

Friday, 17 March 2017 at 17:00

Add to Calendar ▼2017-03-17 17:00:002017-03-17 18:00:00Europe/LondonCharacterization and Separation of U937 Monocytes and U937-Differentiated Macrophages Using 3D Carbon Electrode

Tumor-associated macrophages (TAM), one of the key players in tumor microenvironment, are involved in tumor development and progression in many cancers. Here, we will present carbon electrode dielectrophoresis (carbon-DEP) as a characterization tool to identify and separate U937 monocytes and U937-differentiated macrophages. First, we will differentiate monocytes to obtain macrophages. Next, we will apply carbon-DEP to determine specific crossover frequencies as signatures. Since DEP does not require any pre-labeling for the cells, it will allow direct characterization of cells based on their physical intrinsic properties without altering their genetic and phenotypic properties. Finally, we will verify our results using traditional assays. When we obtain their dielectrophoretic signatures, we will isolate and enrich them for high-throughput biochemical analysis.

Add to Calendar ▼2017-03-16 00:00:002017-03-17 00:00:00Europe/LondonBioMEMS, Microfluidics and Biofabrication: Technologies and