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SELECTBIO Conferences Extracellular Vesicles (EVs: Exosomes and Microvesicles): Research, Diagnostics and Therapeutics Applications

Shannon Holliday's Biography

Shannon Holliday, Associate Professor of Orthodontics and Anatomy & Cell Biology, University of Florida College of Dentistry

Shannon Holliday received his PhD in Cell Biology from the Florida State University followed by post-doctoral work at the National Institutes of Health. He then held postdoctoral and research faculty positions at the Washington University of St. Louis. He is currently Associate Professor in Orthodontics and Anatomy & Cell Biology at the University of Florida College of Dentistry. Holliday has a long-standing interest in the molecular mechanisms by which osteoclasts resorb bone. He has been particularly interested in the vacuolar H+-ATPase (V-ATPase) and its links to glycolytic enzymes which form a proton pumping metabolon, and to the actin cytoskeleton, which his lab showed to be crucial for V-ATPase sorting and trafficking in osteoclasts. Holliday became interested in extracellular vesicles (EVs) as a means by which osteoclasts might regulate osteoblasts during bone remodeling. His lab currently is examining signaling mechanisms involving EVs released by osteoclasts with the goal of using EVs as therapeutics or therapeutic targets in the treatment of bone diseases (osteoporosis, bone cancer) and to facilitate bone regeneration.

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EV Biomarkers from Resorbing Osteoclasts: A Complex Solution?

Thursday, 29 March 2018 at 17:00

Add to Calendar ▼2018-03-29 17:00:002018-03-29 18:00:00Europe/LondonEV Biomarkers from Resorbing Osteoclasts: A Complex Solution?

Recent studies have identified elements of EVs released by osteoclasts (microRNA-214-3p and receptor activator of nuclear factor kappa B) as circulating biomarkers for osteoporosis and psoriatic arthritis.  To identify additional candidates, we collected EVs from osteoclasts that were inactive (cultured on plastic) or resorbing (cultured on bone slices) and performed high-resolution 2D LC/MS analysis.  These data suggest that a number of different types of protein complexes are highly enriched in the EVs from osteoclasts.  Among these, a complex containing subunits of the vacuolar H+-ATPase (V-ATPase) is a strong candidate to serve as a biomarker for bone resorption.  We are currently validating V-ATPase subunit complexes and other protein complexes found in EVs as biomarkers for bone resorption in the gingival crevicular fluid.

Add to Calendar ▼2018-03-28 00:00:002018-03-29 00:00:00Europe/LondonExtracellular Vesicles (EVs: Exosomes and Microvesicles): Research, Diagnostics and Therapeutics