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SELECTBIO Conferences Extracellular Vesicles (EVs: Exosomes and Microvesicles): Research, Diagnostics and Therapeutics Applications

Shannon Holliday's Biography



Shannon Holliday, Associate Professor of Orthodontics and Anatomy & Cell Biology, University of Florida

Shannon Holliday is an Associate Professor of Orthodontics and Anatomy & Cell Biology at the University of Florida. He received his B.S. in Biology from West Virginia Tech, and a PhD in Cell Biology from the Florida State University focused on the crawling motility of nematode sperm. He continued his training as a postdoctoral fellow at the NIH in the lab of Edward D. Korn studying regulation of actin polymerization. During a second postdoc at Washington University in St Louis, he was introduced to osteoclasts and the vacuolar H+-ATPase (V-ATPase) in the laboratory of Stephen L. Gluck. He focused on the V-ATPase and the cytoskeleton in osteoclasts for many years. He became interested in extracellular vesicles (EVs) in 2013, and his group published one of the first articles on regulatory EVs released by osteoclasts in 2016. He is currently seeking strategies to develop new types of bone anabolic therapies based on EVs.

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EV Biomarkers from Resorbing Osteoclasts: A Complex Solution?

Thursday, 29 March 2018 at 17:00

Add to Calendar ▼2018-03-29 17:00:002018-03-29 18:00:00Europe/LondonEV Biomarkers from Resorbing Osteoclasts: A Complex Solution?SELECTBIOenquiries@selectbiosciences.com

Recent studies have identified elements of EVs released by osteoclasts (microRNA-214-3p and receptor activator of nuclear factor kappa B) as circulating biomarkers for osteoporosis and psoriatic arthritis.  To identify additional candidates, we collected EVs from osteoclasts that were inactive (cultured on plastic) or resorbing (cultured on bone slices) and performed high-resolution 2D LC/MS analysis.  These data suggest that a number of different types of protein complexes are highly enriched in the EVs from osteoclasts.  Among these, a complex containing subunits of the vacuolar H+-ATPase (V-ATPase) is a strong candidate to serve as a biomarker for bone resorption.  We are currently validating V-ATPase subunit complexes and other protein complexes found in EVs as biomarkers for bone resorption in the gingival crevicular fluid.


Add to Calendar ▼2018-03-28 00:00:002018-03-29 00:00:00Europe/LondonExtracellular Vesicles (EVs: Exosomes and Microvesicles): Research, Diagnostics and Therapeutics ApplicationsSELECTBIOenquiries@selectbiosciences.com