Conferences \ Circulating Biomarkers World Congress 2017 \ Exosomes and Extracellular Vesicles (EV): Research, Diagnostics and Therapeutics Opportunities \ Agenda \ George Daaboul |
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Automated Proteomic Characterization of Single ExosomesTuesday, 21 March 2017 at 13:30 Add to Calendar ▼2017-03-21 13:30:002017-03-21 14:30:00Europe/LondonAutomated Proteomic Characterization of Single ExosomesSELECTBIOenquiries@selectbiosciences.com Exosome research requires validation of prepared samples that has traditionally been performed using nanoparticle tracking analysis (NTA) combined with proteomic analysis for exosomal proteins. Recently, Flow Cytometry (FC) has been used to combine the sizing and proteomic information about samples under analysis. While a mature tool for large cellular-sized analyses, FC has limitations resulting from the small-size of exosomes, specifically in terms of the number of available surface epitopes and low-signal relative to background levels, especially in complex samples. nanoView Diagnostics has recently developed a label-free visible-light microarray imaging technique that allows multiplexed enumeration and sizing of individual nanovesicles captured on the sensor in a one-step assay direct-from-sample and can work with samples volumes as small as 5 µl. The exosome characterization technology is a high-throughput technique that can improve standardization of exosome preparations and facilitate translation of exosome based liquid biopsies. |