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SELECTBIO Conferences RNA-Seq, Single Cell Analysis & Single Molecule Analysis 2017

Lydia Sohn's Biography

Lydia Sohn, Professor, Department of Mechanical Engineering, University of California, Berkeley

Lydia L. Sohn received her A.B. (Chemistry and Physics, 1988), M.S. (Physics, 1990), and Ph.D. (Physics, 1992) from Harvard University. She was an NSF/NATO postdoc at Delft University of Technology and a postdoc at AT&T Bell Laboratories (1993-1995). Sohn was an Assistant Professor of Physics at Princeton University prior to joining the Mechanical Engineering Dept. at UC Berkeley in 20013. Her work focuses on developing quantitative techniques to probe single cells. Sohn has received numerous awards including the NSF CAREER, Army of Research Young Investigator Award, DuPont Young Professor Award, and a Bakar Fellowship. In 2014, she was one of five winners in the “Identifying Platform Technologies for Advancing Life Sciences Research” competition for her work on Node-Pore Sensing. Most recently, she was elected a Fellow of the American Institute for Medical and Biological Engineering.

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Node-Pore Sensing: A Versatile, Label-Free Method for Screening Single Cells

Thursday, 5 October 2017 at 14:30

Add to Calendar ▼2017-10-05 14:30:002017-10-05 15:30:00Europe/LondonNode-Pore Sensing: A Versatile, Label-Free Method for Screening Single CellsRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,

Flow cytometry is one of the cornerstones of biomedical research and clinical diagnostics.  With its ability to screen individual cells for multiple protein epitopes simultaneously and subsequently identify sub-populations of cells, flow cytometry has had a profound impact in a broad range of areas including immunology, cancer, and regenerative medicine.  While the current state of-the-art is 18 parameters, flow cytometry is often “practically” limited to 6-10 parameters, as emission spectral overlap is of concern and highly complex analysis to decouple such overlap is needed.  We have developed a rapid, label-free method of screening cells for their phenotypic profile, which we call Node-Pore Sensing (NPS).  NPS involves measuring the modulated current pulse caused by a cell transiting a microchannel that has been segmented by a series of inserted nodes.  When segments between the nodes are functionalized with different antibodies corresponding to distinct cell-surface antigens, cell surface-marker identification can be achieved.  When a “contraction” channel through which cells can squeeze is included between the nodes, mechanical phenotyping is possible.  In this talk, I will demonstrate the versatility of NPS and discuss its potential clinical applications—from phenotyping acute myeloid leukemia patient samples to early breast-cancer detection.

Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,