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SELECTBIO Conferences Exosomes and Liquid Biopsies Asia 2017

Charles Lai's Biography

Charles Lai, Assistant Research Fellow, Institute of Atomic and Molecular Sciences, Academia Sinica

Dr. Lai received his PhD from the University of British Columbia, Canada, in 2010, where he focused on the role of gap junctions in brain cancer. He thereafter joined Dr. Xandra Breakefield’s laboratory for his postdoctoral training and Instructor position in the Department of Neurology at Massachusetts General Hospital and Harvard Medical School. He served as an Assistant Professor of Biomedical Engineering at National Tsing Hua University from 2015 to 2017, and is currently an Assistant Research Fellow at the Institute of Atomic and Molecular Sciences in Academia Sinica of Taiwan, similar to the Max Planck Institutes of Germany. His current work focuses on developing methods to enable molecular imaging and application of nanosized extracellular vesicles including exosomes.

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From Seeing to Believing: Visualization and Tracking of Extracellular Vesicles

Monday, 19 June 2017 at 14:30

Add to Calendar ▼2017-06-19 14:30:002017-06-19 15:30:00Europe/LondonFrom Seeing to Believing: Visualization and Tracking of Extracellular VesiclesExosomes and Liquid Biopsies Asia 2017 in Taipei, TaiwanTaipei,

Extracellular vesicles (EVs), including exosomes and microvesicles, are nanosized membrane- bound vesicles released in abundance by cancer cells. These EVs are capable of delivering a select subset of proteins and RNAs designed to promote tumor growth and metastases. However, the exact time, location (i.e. spatiotemporal properties) and cargo delivery capacity of EVs remained unknown due to a lack of available methods to detect them. Here we engineered bioluminescent and fluorescent reporter systems which allow EV visualization in real-time both in vitro and in vivo. The multimodal bioluminescent reporter labels EVs with a membrane-bound variant of Gaussia luciferase fused to a biotin acceptor peptide, thereby enabling whole-animal multimodal imaging of nanoscale EVs, as well as pharmacokinetics analysis of EVs. We next developed the bifunctional fluorescent EV/EV-RNA reporters to study multiple EV populations with subcellular image resolution. By multiplexing the fluorescent and bioluminescent EV reporters, we further elucidated the rapid dynamics of tumor EV uptake and translation of EV-delivered cargo mRNAs in cancer cells. These novel molecular imaging platforms revealed that EV mediates a dynamic and multidirectional communication between cells with delivery of functional mRNA, thereby manipulating its recipient cells at neighboring and distal sites.

Add to Calendar ▼2017-06-19 00:00:002017-06-20 00:00:00Europe/LondonExosomes and Liquid Biopsies Asia 2017Exosomes and Liquid Biopsies Asia 2017 in Taipei, TaiwanTaipei,