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SELECTBIO Conferences RNA-Seq, Single Cell Analysis & Single Molecule Analysis 2017

Muriel Breteau's Biography

Muriel Breteau, Technical Applications Specialist, Dolomite Bio

Dr Muriel Breteau holds a master of Science in Biotechnology from École Supérieure de Biotechnologie, France and a Doctorate in Microbiology from the University of Warwick. During her PhD she worked on the development of phage therapy, which involved the isolation and characterisation of suitable bacteriophages phenotypically and genetically. At that time she became interested in the potential of molecular biology and genomics for identifying markers and other characteristics with possible therapeutic applications in bacteria or phages as well as in eukaryotic cells. Muriel Breteau is an Applications Biologist for Dolomite Bio, part of the Blacktrace group of companies. She is responsible for identifying and developing applications and optimising technologies for a range of single-cell microfluidic encapsulation products for single cell profiling.

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Revolutionizing Single Cell Research with High Throughput Single Cell RNA-Seq

Thursday, 5 October 2017 at 16:00

Add to Calendar ▼2017-10-05 16:00:002017-10-05 17:00:00Europe/LondonRevolutionizing Single Cell Research with High Throughput Single Cell RNA-SeqRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,

Analyzing single cells has enabled researchers to understand the complexity of biologic systems and gain vital information and data which is key to understanding many diseases and immunity. However, until recently, methods involved bulk analysis, causing vital information at the single cell level to be lost.

With the emergence of scRNA-Seq, this has offered an exciting, cutting edge technique that for the first time gives unprecedented insight into the expression of thousands of single cells. This method has revolutionised the way we analyse single cells and enables additional understanding into tissues at the level of individual cells.

This presentation will investigate:

  • Current methods to perform single cell RNA-Seq including the effective use of microfluidic droplet technology to enable access to the transcriptome of thousands of single cells in a high throughput manner
  • Advantages and benefits of using the drop-seq protocol for scRNA-Seq
  • The development of new protocols and application using flexible single cell tools including profiling natively paired TCRs, isolating antibody coding sequences, encapsulating cells in hydrogels or encapsulating expression libraries for FACS sorting

Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,