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SELECTBIO Conferences Exosomes & Liquid Biopsies Europe 2018

Esther Nolte-‘t Hoen's Biography

Esther Nolte-‘t Hoen, Assistant Professor, Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University

Dr. Esther Nolte-‘t Hoen did her PhD (2003) on regulation of T cell responses in the lab of Professor Wauben at Utrecht University, The Netherlands. At this early stage, she discovered that T cells can modulate the function of antigen presenting cells by transferring proteins via small extracellular vesicles. As a post-doctoral fellow in Prof. Daniel Davis’ laboratory at Imperial College London, Nolte-‘t Hoen further explored the cell biology of immune cell interactions using state-of-the-art microscopical techniques. After a second post-doc at Utrecht University, she was appointed Assistant Professor in 2013. Her research focuses on the role of extracellular vesicles in communication between immune cells at steady state and during microbial infection. Within this topic, she puts strong focus on technical aspects of extracellular vesicle purification, methods for high-throughput individual vesicle analysis, the RNA content of extracellular vesicles, and the interplay between vesicles and viruses. Nolte-‘t Hoen was awarded a European Research Council Starting Grant (1500 k€) in 2013 to explore parallel mechanisms underlying the formation of viruses and extracellular vesicles. Nolte-‘t Hoen is an active member of the International Society of Extracellular Vesicles and in 2015 organized an international research seminar for this society on how the function of extracellular vesicle-associated RNA can be unraveled.

Esther Nolte-‘t Hoen Image

Interpreting Extracellular Vesicles as Snapshots of Cells

Thursday, 25 October 2018 at 10:30

Add to Calendar ▼2018-10-25 10:30:002018-10-25 11:30:00Europe/LondonInterpreting Extracellular Vesicles as Snapshots of CellsExosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The

Our research focuses on the role of EV in communication between cells at steady state and during immune activation or microbial infection. Using high-resolution flow cytometric analysis of single EV, we previously showed that EV populations are highly heterogeneous. Our recent work on EV released by immune cells and virus-infected cells indicates that both spatial and temporal elements drive EV heterogeneity. This has implications for interpreting EV as snapshots of cells. Recognition and in-depth analysis of EV heterogeneity can facilitate the search for refined EV-based biomarkers. Moreover it can help to dissect differences in the formation and function of distinct EV populations. Examples will be shown on how environmental triggers can lead to changes in the type of released EV and their small non-coding RNA content. Additionally, it will be explained how advanced flow cytometry-based technologies can be applied to study EV release dynamics and to isolate EV subpopulations for downstream functional analysis.

Add to Calendar ▼2018-10-24 00:00:002018-10-26 00:00:00Europe/LondonExosomes and Liquid Biopsies Europe 2018Exosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The