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SELECTBIO Conferences RNA-Seq, Single Cell Analysis & Single Molecule Analysis 2017

Ermelinda Porpiglia's Biography

Ermelinda Porpiglia, Life Science Research Associate, Stanford University School of Medicine

Dr. Ermelinda Porpiglia received her Ph.D. at the University of Massachusetts Medical School and subsequently conducted post-doctoral work at the Department of Microbiology and Immunology, Stanford University School of Medicine.

Ermelinda Porpiglia Image

High-Resolution Lineage Mapping of Myogenesis in vivo

Friday, 6 October 2017 at 07:30

Add to Calendar ▼2017-10-06 07:30:002017-10-06 08:30:00Europe/LondonHigh-Resolution Lineage Mapping of Myogenesis in vivoRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,

Muscle regeneration is a dynamic process during which the state and identity of the cells involved changes over time. Adult muscle stem cells are the driving force in skeletal muscle repair and regeneration. Mostly quiescent in healthy individuals, they become activated in response to muscle injury. While the phenotypic identity of muscle stem cells has been previously established, muscle progenitor cells, which are at a stage in between the stem cell and the mature functional myogenic cell, have not yet been identified in vivo. Progenitor cells hold enormous potential as a platform to study the cellular behavior and molecular control of stem cell fate, to identify novel therapeutic targets for muscle diseases, and to develop cell therapy applications for regenerative medicine. However, a major roadblock in their identification has been a lack of tools to resolve cellular heterogeneity in skeletal muscle, underscoring the importance of single-cell studies. We have capitalized on single-cell mass cytometry (CyTOF), a transformative technology that allows the discovery of novel subsets within a complex cell population, to capture stem cell fate decisions in vivo. We discovered novel cell surface markers that define a myogenic progression in vivo, by combining a high-throughput flow cytometry screen with CyTOF analysis of skeletal muscle. Importantly, using new marker sets we identified stem cells and previously unrecognized progenitor cell populations. We showed by lineage tracing experiments that these progenitor populations originate from muscle stem cells and exhibit distinct regenerative potential in vivo. Moreover, high dimensional CyTOF analysis in response to acute injury revealed the cellular and molecular dynamics of muscle regeneration at a level of resolution not previously possible.

Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island,