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SELECTBIO Conferences Lab-on-a-Chip & Microfluidics Europe 2019

Charalampos Tzivelekis's Biography



Charalampos Tzivelekis, Researcher, Newcastle University

Babis Tzivelekis is a researcher in the School of Engineering at Newcastle University (UK). He is researching the rapid manufacturing of microfluidic devices and instrumentation for DNA diagnosis with innovative, low-cost stereolithography 3D printing and high-precision machining techniques. During his PhD he has worked extensively on the design, manufacture, characterisation and optimisation of polymer lab-on-chip platforms to process DNA based assays for Polymerase Chain Reaction (PCR). His research is co-funded by the Engineering and Physical Sciences Research Council (EPSRC) Centre for Doctoral Training in Additive Manufacturing and QuantuMDx, Ltd.

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Fabrication via DLP-SLA and Characterization of Shuttle Flow 2-step PCR Microfluidic Cartridges

Wednesday, 19 June 2019 at 17:30

Add to Calendar ▼2019-06-19 17:30:002019-06-19 18:30:00Europe/LondonFabrication via DLP-SLA and Characterization of Shuttle Flow 2-step PCR Microfluidic CartridgesLab-on-a-Chip and Microfluidics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com

DLP® Stereolithography (SLA) as a high-resolution 3D printing process offers a low-cost alternative for prototyping of polymer microfluidic devices, compared to common fabrication methods. Here, we introducespecific processing routes of DLP SLA infabrication of microfluidic devices suitable for Polymerase Chain Reaction (PCR). The influence of printed polymer on DNA yield during PCR was assessed in mock reactions, and a fabrication protocol for cartridges with monolithic 2.5D micro-channels evaluated in the production of disposable flow-through PCR devices. Those were interfaced with a purpose-built PCR thermocycler and evaluated in-situ by amplification of a 75 bp target from a plasmid DNA template. PCR inhibition sources were further analyzed with qPCR. Considering the low unit cost per device and the capability to endow further functionality on printed devices through surface modification, we further examined a hydrophobic tuning protocol of prints and analyzed its performance with qPCR. We finally summarize the advantages of DLP SLA and characterize its potential as a future fabrication technique of lab-on-chip devices.


Add to Calendar ▼2019-06-18 00:00:002019-06-19 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2019Lab-on-a-Chip and Microfluidics Europe 2019 in Rotterdam, The NetherlandsRotterdam, The NetherlandsSELECTBIOenquiries@selectbiosciences.com