Shopping Cart (0)
My Account

Shopping Cart
SELECTBIO Conferences The Space Summit 2019

Arun Sharma's Biography



Arun Sharma, Senior Research Fellow, Cedars-Sinai Medical Center

Arun Sharma, PhD is a Senior Research Fellow at the Cedars-Sinai Medical Center.

Prior to this he was postdoctoral research fellow at the Harvard Medical School Department of Genetics. His research focuses on the applications of induced pluripotent stem cells (iPSCs) for studying cardiovascular biology, modeling diseases “in a dish”, and high-throughput screening of drug toxicity. As a graduate student at Stanford University, he led an effort to send a sample of hiPSC-derived cardiomyocytes to the International Space Station to investigate the effects of microgravity on human heart cell function.

Arun Sharma Image

Modeling the Effects of Spaceflight on the Human Heart Using Stem Cell-derived Cardiomyocytes

Monday, 14 October 2019 at 09:45

Add to Calendar ▼2019-10-14 09:45:002019-10-14 10:45:00Europe/LondonModeling the Effects of Spaceflight on the Human Heart Using Stem Cell-derived CardiomyocytesThe Space Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

With extended stays aboard the International Space Station (ISS) becoming commonplace as humanity prepares for exploration-class space missions, the need to better understand the effects of microgravity on cardiac function during spaceflight is critical. However, primary human heart tissues, which would be useful for in vitro studies on heart function, are difficult to obtain and maintain. As a model system, we utilized cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) to study the effects of microgravity on human cardiac function and gene expression at the cellular level. We derived hiPSCs from three healthy volunteers and produced hiPSC-CMs using a high-efficiency differentiation protocol. We cultured hiPSC-CMs in a microgravity environment aboard the ISS for approximately one month, during which weekly media changes were conducted. We analyzed the gene expression, structure, and function of space-flown and groundside control hiPSC-CM samples using RNA-sequencing, immunofluorescence, calcium imaging, and contractility assessment. This study represents the first time that hiPSC technology has been used to study the effects of spaceflight on human cardiomyocyte function and demonstrates that microgravity affects human cardiomyocyte function on the cellular level.


Add to Calendar ▼2019-10-14 00:00:002019-10-15 00:00:00Europe/LondonThe Space Summit 2019The Space Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com