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SELECTBIO Conferences 3D-Culture & Organoids 2019

Feng Li's Biography

Feng Li, Senior Scientist, Development, Corning Life Sciences

Dr. Feng Li is a Senior Development Scientist of Corning Life Sciences and expert cell biologist with over 12 year experience in biotechnology industry. He received his Ph.D. from University of Colorado and postdoctoral training at Princeton University. Prior to joining Corning he worked in the area of cell differentiation and large scale production process of blood cells from pluripotent stem cells. In his current role, he has been involved in market research, technology evaluation, and product and manufacture process development. In recent years, he has focused on spheroid models using primary human hepatocytes and Corning 3D technologies for assay development and applications in liver toxicity testing and disease modeling.

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3D Human Liver Spheroids for In Vitro Liver Toxicity Testing

Tuesday, 15 October 2019 at 09:30

Add to Calendar ▼2019-10-15 09:30:002019-10-15 10:30:00Europe/London3D Human Liver Spheroids for In Vitro Liver Toxicity Testing3D-Culture and Organoids 2019 in Coronado Island, CaliforniaCoronado Island,

3D liver spheroids can be made from primary human hepatocytes (PHH) with or without other liver cell types. For 3D liver spheroid assay development, we have evaluated the impact of donor variations on spheroid formation and stability in vitro. Our results show that PHH lots need to be pre-tested for 3D spheroid culture and there exist variations in spheroid formation time and stability among different donor lots. Using a large set of 100 compounds, PHH liver spheroids are shown to significantly improve the prediction accuracy of drug induced liver toxicity comparing to conventional system. Furthermore, 3D spheroid culture qualified PHH can be used to generate co-culture liver spheroids with other liver cell types such as Primary human Kupffer cells. As 3D liver spheroid models are being adopted by more industry users, we’d like to share our experience working with liver spheroids and toxicity test results to facilitate a conversation on how to standardize 3D liver spheroid models for in vitro assays.

Add to Calendar ▼2019-10-14 00:00:002019-10-15 00:00:00Europe/London3D-Culture and Organoids 20193D-Culture and Organoids 2019 in Coronado Island, CaliforniaCoronado Island,