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SELECTBIO Conferences EV-based Diagnostics, Delivery & Therapeutics

Andries Zijlstra's Biography



Andries Zijlstra, Associate Professor of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center and Vanderbilt Ingram Cancer Center

The experimental focus of my laboratory is on understanding the modifications of cell adhesion mechanisms that promote tumor progression and defining their clinical relevance. We have two decades of experience using in vitro and in vivo pre-clinical models of oncology and work closely with physicians to frame experiments within a clinically relevant context. Specific projects within the laboratory include: a) structure-function analysis of Activated Leukocyte Cell Adhesion Molecule (ALCAM) and the tetraspanin CD151 in the context of cell adhesion and migration, b) the biogenesis of extracellular vesicles (EVs) and their contribution to cell motility and c) developing biomarkers of malignant progression in urogenital cancers using molecular indicators of cell motility. The latter makes use of high density multiplexing in tissues and liquid biopsy-style approaches for proteins and EVs in plasma and/or urine from cancer patients and deploys computational strategies (i.e. dimensional reduction) we developed to accomplish histological detection of pathological mechanisms in patient specimens. We leverage correlations between clinical specimens and experimental models to understand how specific molecular processes define the outcome of our cancer patients. My laboratory has been fortunate to collaborate with Drs. Di Vizio and Irish to establish a novel flowcytometry method that deconvolves the complexity of EV populations in biological fluids. Our publication record demonstrates our expertise in investigating molecular mechanisms of motility ranging from cell adhesion and matrix deposition to nuclear membrane instability and EV biogenesis.

Andries Zijlstra Image

A Multi-Dimensional Approach to EV Flow Cytometry

Monday, 17 February 2020 at 14:00

Add to Calendar ▼2020-02-17 14:00:002020-02-17 15:00:00Europe/LondonA Multi-Dimensional Approach to EV Flow CytometryEV-based Diagnostics, Delivery and Therapeutics in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

Flow cytometry has proven to be very promising method for Single-EV analysis. Unfortunately, the heterogeneity of EV populations prevents the application of the single-particle classification strategy derived from conventional flow cytometry of intact cells fails in our attempts to resolve distinct classes of EVs. In simple terms, the small size of most EVs prevents the incorporation of all protein markers that define a uniform class of EVs. Consequently, EVs generated through the same biogenesis pathway from the same donor cells, could contain vastly different cargo. Conversely, restricting EV classification only to a size range is equally limiting. To improve EV sub-classification and enable the identification of biologically-significant alterations in EV populations in response to alterations in physiological or pathological states of the donor cell/tissue, we have developed a multi-dimensional approach to EV flow cytometry that attempts to cluster EVs into subpopulations on the basis of many, rather than single parameters.


Add to Calendar ▼2020-02-17 00:00:002020-02-18 00:00:00Europe/LondonEV-based Diagnostics, Delivery and TherapeuticsEV-based Diagnostics, Delivery and Therapeutics in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com