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SELECTBIO Conferences 2D-to-3D Culture and Organoids 2020

Yu-Chi Chen's Biography

Yu-Chi Chen, Scientist, Bioprinting Group, NCATS

Yu-Chi Chen is a high-content image scientist in the 3D Tissue Bioprinting group where she is developing high-content cell imaging assays and image analysis methods applied to 3D cellular models. Yu-Chi has a M.S in Human Genetics and she has worked as Research Scientist both in academia and pharmaceutical industry.

Yu-Chi Chen Image

Phenotypic Machine Learning in High Content Imaging Screening with Organoids

Thursday, 20 August 2020 at 16:00

Add to Calendar ▼2020-08-20 16:00:002020-08-20 17:00:00Europe/LondonPhenotypic Machine Learning in High Content Imaging Screening with Organoids2D-to-3D Culture and Organoids 2020 in Boston, USABoston,

In the last few years, there has been a fast increase in the use of 3D cellular models as physiological relevant assays for drug discovery and development.  The use of U-bottom plates has been widely used for growing 3D spheroids because they facilitate spheroid formation in a scalable and reproducible manner to enable large scale compound screening.  However, the U-bottom shape of the wells in these plates limits the use of high-resolution imaging (>20X) and high-content screening mainly due to light diffraction.  Therefore, the assays used for screening with spheroids have mostly been restricted to simple readouts such as cell viability using standard well-based assays, or high content assays measuring total fluorescence intensity.  As more complex spheroids and organoids models are developed for disease modeling, there is an increased need to be able to quantitate the effects of compounds in different cell types, sub-cellular biomarkers and phenotypes within these 3D systems.  Here we will discuss the development of a 1536-well 3D HCS assay platform that enables the generation of high-resolution sub-cellular images coupled with a Phenotypic machine learning and 3D segmentation analysis within 3D spheroids that enables the implementation of 3D High Resolution Imaging Screening.

Add to Calendar ▼2020-08-19 00:00:002020-08-20 00:00:00Europe/London2D-to-3D Culture and Organoids 20202D-to-3D Culture and Organoids 2020 in Boston, USABoston,