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SELECTBIO Conferences Innovations in Microfluidics & SCA 2021

Claude Dufresne's Biography

Claude Dufresne, CEO, axiVEND LLC

Dr. Dufresne, CEO of axiVEND, is a scientist and entrepreneur. Trained as an organic chemist, with degrees from McGill and the University of Alberta, he spent most of his career at Merck, gaining experience in the fields of laboratory automation, biochemistry, and pharmacology. For the last 8 years, he has transitioned to a role on the provider side of the Life Sciences community, bringing a unique blend of expertise to help identify solutions to the diagnostics industry. He has developed valuable know-how in the topics of microarrays, biosensors and biochips, and ultra-low volume, high precision liquid handling. From a commercial perspective, the founding principle of axiVEND is based on transparent communications, hence the company name from the Greek ‘axios’ (truthfulness).

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The Simultaneous Determination of Biomolecules Binding Affinities On Multiple Varied Immobilization Surfaces

Thursday, 18 March 2021 at 16:30

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In many microfluidics devices, biomolecules are captured by pre-deposited proteins in channels, reaction chambers, or on microarrays. The correct immobilization of these proteins is critical to obtain the best device performance.  While many polymeric materials exist for protein immobilization, it is often quite tedious to go through a selection process.  Beyond binding capacity as a classic factor, often the orientation and functional ligand activity are more important.

This talk will present a method by which multiple polymers can be evaluated in parallel, in the same experiment.  Dozens of candidate surface binding polymeric materials are spotted on a Si/SiO2 chip, followed by spot-on-spot protein functionalization.  Target protein capture is then observed by interferometric reflectance. This allows for binding curves to be generated for each protein/surface polymer pair, in parallel, and under the same conditions.  The results are invaluable in assessing various polymeric formulations for optimal presentation of the capture molecule and its ensuing target molecule binding properties.

Since the underlying device material is covered with the binding polymer, the method described is applicable to a wide range of applications.

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