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SELECTBIO Conferences Extracellular Vesicles 2023: Technologies, Biomarker Cargo & Diagnostics

Robert Thacker's Biography



Robert Thacker, Imaging Sales and Applications Specialist, Cytek Biosciences

Dr. Robert Thacker is an imaging specialist for Cytek Biosciences, working specifically with the Amnis imaging flow cytometry systems. Rob is currently responsible for managing new imaging instrument sales and post-sales support in 18 states in the Midwest and Mid-Atlantic regions and 2 Canadian provinces. Major accounts include the National Institutes of Health in Bethesda, Maryland. He started his current position in 2014, after supporting the technology for 2 years as a field applications scientist.

Rob has an undergraduate degree in microbiology (BS, Brigham Young University 2004), but focused on dendritic cell biology and how dendritic cells activate various types of immune responses for his graduate and post-doctoral work (PhD, University of Cincinnati, 2008; Post-doctoral training, Cincinnati Children’s Hospital, 2008-2012). Rob’s graduate research investigated the role of fibrinogen in immune cell activation, cancer metastasis, and the enhancement of vaccine formulations. Post-doctoral research studied the mechanisms behind the presentation of both viral and cell-associated antigens. Utilizing Amnis imaging in flow during his post-doctoral research, Rob specifically investigated the uptake, internalization and trafficking of apoptotic tumor cells within various dendritic cell subsets to understand how a better and more specific anti-tumor response might be achieved. It was this work that led him to be hired by MilliporeSigma in 2012 as an Amnis applications scientist, a position that evolved into his current role as imaging specialist for the same technology that now is a part of Cytek Biosciences.

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Extracellular Vesicle Detection and Calibration on the Cytek® Amnis® CellStream® and Cytek® Amnis® ImageStream® Systems

Wednesday, 26 July 2023 at 15:00

Add to Calendar ▼2023-07-26 15:00:002023-07-26 16:00:00Europe/LondonExtracellular Vesicle Detection and Calibration on the Cytek® Amnis® CellStream® and Cytek® Amnis® ImageStream® SystemsExtracellular Vesicles 2023: Technologies, Biomarker Cargo and Diagnostics in Orlando, FloridaOrlando, FloridaSELECTBIOenquiries@selectbiosciences.com

The quantification and characterization of extracellular vesicles is challenging due to their size. High magnification microscopy can analyze EVs, but has very low throughput. Flow cytometry is currently the only method building in rigor and reproducibility for single EV measurements, but older PMT-based flow cytometers have limitations in detecting EVS due to low sensitivity, cutting out the smallest of particles. Flow cytometry systems that use a charge coupled device (CCD)-camera have greatly enhanced sensitivity and increase the limit of detection. Today’s presentation will focus on two such CCD based systems – the Cytek® Amnis® CellStream® and Cytek® Amnis® ImageStream®  - and share results  from studies demonstrating the low limit of detection and the importance of calibration for more accurate sizing standardization of protocols within the field.


Add to Calendar ▼2023-07-26 00:00:002023-07-27 00:00:00Europe/LondonExtracellular Vesicles 2023: Technologies, Biomarker Cargo and DiagnosticsExtracellular Vesicles 2023: Technologies, Biomarker Cargo and Diagnostics in Orlando, FloridaOrlando, FloridaSELECTBIOenquiries@selectbiosciences.com