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SELECTBIO Conferences Lab-on-a-Chip and Microfluidics Europe 2020

Hsueh-Chia Chang's Biography

Hsueh-Chia Chang, Bayer Professor of Engineering, University of Notre Dame

H-C (Chia) Chang is a leader in electrokinetics, an important micro/nanofluidic technology for biochip platforms in future disease diagnostic products. His approach combines insightful theoretical analysis with simple but creative experiments to uncover new electrokinetic phenomena or to verify speculated ones. These new phenomena then led to inventions in new molecular diagnostics that use non-equilibrium electrokinetics and field-focusing physics to dramatically improve the performance of equilibrium probe-based assays without external fields. He and his PhD and postdoc students are inventors of 10 Notre Dame patents and 6 provisional patents, the largest IP output from any lab at Notre Dame. Five technologies have been licensed by startups near Notre Dame. One startup FCubed LLC will be going public this year. Another startup AgenDx has just licensed another of his technologies. Since 2000, 18 PhD and post-doc students of the Chang laboratory have embarked on academic careers as tenure-track professors at Chemical Engineering, Mechanical Engineering, Electrical Engineering, Food Science, Chemistry departments in 5 continents. Chia is the coauthor of a seminal book on Electrokinetics and he is the Founding Editor of Biomicrofluidics, the first American Institute of Physics journal in biology. He has published over 270 articles with 13,000 citations and an H-index of 63.

Hsueh-Chia Chang Image

Fractionation and Isolation of Exosomes and other Nanocarriers of Molecular Biomarkers

Thursday, 10 September 2020 at 10:00

Add to Calendar ▼2020-09-10 10:00:002020-09-10 11:00:00Europe/LondonFractionation and Isolation of Exosomes and other Nanocarriers of Molecular BiomarkersLab-on-a-Chip and Microfluidics Europe 2020 in Rotterdam, The NetherlandsRotterdam, The

It is now known that most proteins and nucleic acid biomarkers in the blood and other physiological fluids are encased in a large variety of nanodimension carriers, which are lipid vesicles or protein-nucleic acid complexes.  There is also evidence that such vesicles and complexes inherit the membrane biomarkers of their cells of origin.  Moreover, even within the same cell, different molecules are packaged into different nanocarriers before they are released.  Consequently, whose-sale assay of all the biomarkers would not allow us to accurately quantify their irregular expression in specific cells, such as cancer cells from a particular organ. Current nanocarrier fractionation technologies by ultra-centrifugation, precipitation, size-exclusion, nanofiltration and immunocapture, however, have too low a yield and selectivity,  because of lysing, aggregation, non-selective isolation and other issues. Instead, we report several new fractionation and isolation technologies, based on their size, charge and surface proteins. The key issues are analyzed and resolved, with new inventions based on fundamental principles in nanofluidics, electrokinetics and paramagnetics, to offer significantly higher yield (>90%), selectivity (>95%) and throughput (<1 hour).  We will also report several new molecular sensors that will be integrated with the nanocarrier fractionation technologies for rapid (<30 min) and low-copy number (~100) quantification of multiple molecular biomarkers from the nanocarriers. Our overarching goal is to build a turn-key platform for profiling of multi-biomarkers in a large number of samples.

Add to Calendar ▼2020-09-09 00:00:002020-09-10 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics Europe 2020Lab-on-a-Chip and Microfluidics Europe 2020 in Rotterdam, The NetherlandsRotterdam, The