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SELECTBIO Conferences Lab-on-a-Chip & Microfluidics World Congress 2017

Hsueh-Chia Chang's Biography



Hsueh-Chia Chang, Bayer Professor of Engineering; Director, Center for Microfluidics and Medical Diagnostics, University of Notre Dame

Chia Chang received his BS and PhD degrees in Chemical Engineering from Caltech and Princeton. His group's research in electrokinetics and microfluidics has produced several on-chip micro-pumping, mixing, cell manipulation, electrical and plasmonic molecular sensing technologies. Some of them are being commercialized by three start-ups. One company that works closely with the Notre Dame Haiti program, FCubed LLC, http://www.fcubed.biz/, now employs 17 employees and has attracted more than $10 million in investment. Over 20 of Chia's group alumni now hold faculty positions over all six continents. He is the founding and chief editor of Biomicrofluidics, an American Institute of Physics journal.

Hsueh-Chia Chang Image

PCR-Free MicroRNA Quantification Based on Ion-Selective Nanoporous Membranes and Nanopores

Monday, 2 October 2017 at 17:15

Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com

We report an integrated biochip platform that can identify and quantify low copy numbers of microRNA biomarkers in a heterogeneous physiological sample like blood, saliva or urine. This quantification assay is done without PCR amplification, reporter labeling,  extensive off-chip pretreatment and expensive optical sensors.  Consequently, it does not introduce PCR/ligation bias and limits analyte loss during pretreatment.  The main components of the integrated biochips are nanoporous membranes and solid-state nanopores with pore radii smaller than the nm-scale Debye length. We use the ion concentration and charge polarization features of the ion-selective membranes to control the on-chip ionic strength, actuate pH by splitting water, lyse exosomes and isolate/concentrate the target molecules. The final nanopore sensor or sensor array utilizes surface modification and pore geometry to preferentially delay the translocation time of the target microRNAs to achieve single-molecule identification and quantification. The integrated chip achieves a translocation frequency (throughput) that is at least one hundred times higher than any literature or commercial nanopore technology.

PCR-Free MicroRNA Quantification Based on Ion-Selective Nanoporous Membranes and Nanopores

Monday, 2 October 2017 at 17:15

Add to Calendar ▼SELECTBIOenquiries@selectbiosciences.com

We report an integrated biochip platform that can identify and quantify low copy numbers of microRNA biomarkers in a heterogeneous physiological sample like blood, saliva or urine. This quantification assay is done without PCR amplification, reporter labeling,  extensive off-chip pretreatment and expensive optical sensors.  Consequently, it does not introduce PCR/ligation bias and limits analyte loss during pretreatment.  The main components of the integrated biochips are nanoporous membranes and solid-state nanopores with pore radii smaller than the nm-scale Debye length. We use the ion concentration and charge polarization features of the ion-selective membranes to control the on-chip ionic strength, actuate pH by splitting water, lyse exosomes and isolate/concentrate the target molecules. The final nanopore sensor or sensor array utilizes surface modification and pore geometry to preferentially delay the translocation time of the target microRNAs to achieve single-molecule identification and quantification. The integrated chip achieves a translocation frequency (throughput) that is at least one hundred times higher than any literature or commercial nanopore technology.


Add to Calendar ▼2017-10-02 00:00:002017-10-04 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics World Congress 2017Lab-on-a-Chip and Microfluidics World Congress 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com