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SELECTBIO Conferences Microfluidics for Circulating Biomarkers Summit 2019

Hsueh-Chia Chang's Biography



Hsueh-Chia Chang, Bayer Professor of Engineering, University of Notre Dame

H-C (Chia) Chang is a leader in electrokinetics, an important micro/nanofluidic technology for biochip platforms in future disease diagnostic products. His approach combines insightful theoretical analysis with simple but creative experiments to uncover new electrokinetic phenomena or to verify speculated ones. These new phenomena then led to inventions in new molecular diagnostics that use non-equilibrium electrokinetics and field-focusing physics to dramatically improve the performance of equilibrium probe-based assays without external fields. He and his PhD and postdoc students are inventors of 10 Notre Dame patents and 6 provisional patents, the largest IP output from any lab at Notre Dame. Five technologies have been licensed by startups near Notre Dame. One startup FCubed LLC will be going public this year. Another startup AgenDx has just licensed another of his technologies. Since 2000, 18 PhD and post-doc students of the Chang laboratory have embarked on academic careers as tenure-track professors at Chemical Engineering, Mechanical Engineering, Electrical Engineering, Food Science, Chemistry departments in 5 continents. Chia is the coauthor of a seminal book on Electrokinetics and he is the Founding Editor of Biomicrofluidics, the first American Institute of Physics journal in biology. He has published over 270 articles with 13,000 citations and an H-index of 63.

Hsueh-Chia Chang Image

Multiplex Liquid Biopsy Platform for Fractionation of Heterogeneous Vesicles and Precision Analyses of Their RNA Cargo

Thursday, 10 October 2019 at 09:00

Add to Calendar ▼2019-10-10 09:00:002019-10-10 10:00:00Europe/LondonMultiplex Liquid Biopsy Platform for Fractionation of Heterogeneous Vesicles and Precision Analyses of Their RNA CargoMicrofluidics for Circulating Biomarkers Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

Extracellular RNAs in blood are promising circulating biomarkers.  However, as they can be easily degraded, extracellular RNAs must form complexes with proteins or are encapsulated by liposomes, exosomes or microvesicles to remain stable.  As a result, stable RNA biomarkers, like mRNA and miRNA, are carried by a heterogeneity of nanocarriers in blood with wide size, electrophoretic mobility and isoelectric point distributions.  Due to their different biogenesis, these carriers have specific surface proteins and are often selective in their choice of RNA cargoes.  To offer more precise quantification of the circulating RNA biomarkers, we have developed a suite of microfluidic modules that can fractionate the carriers by size, surface proteins and isoelectric point.  Size-based separation is achieved by an asymmetric nanoporous membrane whose conic pores reduce hydrodynamic resistance and vesicle fusion.  Extensive rinsing can be carried out to remove free-floating contaminating proteins like albumin.  We also developed several lysing modules that can lyse the vesicles and dissociate the complexes non-chemically, such that low-yield extraction can be eliminated as an intermediate step.  We integrate these modules with our membrane sensor, digital PCR module and nanopore sensor for RNA identification and quantification to achieve an integrated multiplexed platform for precise profiling of RNA cargoes in fractionated carriers.  Some of the upstream modules can also be integrated into a pretreatment platform for NGS.


Add to Calendar ▼2019-10-10 00:00:002019-10-10 00:00:00Europe/LondonMicrofluidics for Circulating Biomarkers Summit 2019Microfluidics for Circulating Biomarkers Summit 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com