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SELECTBIO Conferences 3D-Culture, Organoids and Organs-on-Chips 2021

Terry Riss's Biography

Terry Riss, Senior Product Manager, Cell Health, Promega Corporation

Dr. Terry Riss started the Cell Biology program at Promega Corporation in 1990 and held several R&D and Project Management positions since. Dr. Riss managed development of cell viability, cytotoxicity, apoptosis, and protease assay systems and lead efforts to identify and promote multiplexing of cell-based assays to determine the mechanism of cell death. Dr. Riss now serves as Senior Product Manager, Cell Health involved in outreach educational training activities including validating assay systems applied to 3D cell culture models. Dr. Riss also serves as an editor of the In Vitro Cell-Based Assays section of the Assay Guidance Manual hosted by The National Center for Advancing Translational Sciences (NCATS) at the NIH.

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Live-Cell Kinetic Assays of 3D Cultures to Monitor Cell Health and Metabolite Levels Used to Select Samples For Further Analysis

Tuesday, 23 March 2021 at 09:00

Add to Calendar ▼2021-03-23 09:00:002021-03-23 10:00:00Europe/LondonLive-Cell Kinetic Assays of 3D Cultures to Monitor Cell Health and Metabolite Levels Used to Select Samples For Further Analysis3D-Culture, Organoids and Organs-on-Chips 2021 in

With the increased use of physiologically relevant models comes the need for sensitive assay methods to monitor health of cells cultured in a 3D environment. Live-cell kinetic assays are available to interrogate 3D culture models and report the number of live cells, dead cells, apoptotic cells, or key metabolite levels remaining in the medium. Because the live-cell kinetic assays do not kill the cells, data from real-time monitoring of changes in viability or metabolite utilization can be set as a threshold to cherry pick a subset of individual samples for more time consuming image recording and computational analysis using high content screening. We will describe how we use the power of luminescence detection to overcome the limitations in sensitivity and create live-cell kinetic assays to monitor 3D culture models.

Add to Calendar ▼2021-03-22 00:00:002021-03-23 00:00:00Europe/London3D-Culture, Organoids and Organs-on-Chips 20213D-Culture, Organoids and Organs-on-Chips 2021 in