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SELECTBIO Conferences Exosomes & Liquid Biopsies Europe 2018

Niek Dekker's Biography

Niek Dekker, Principal Scientist, Discovery Sciences, AstraZeneca

Trained as a biochemist with a PhD in enzymology and postdoctoral experience on molecular biology and structural biology. Expertise on structure-function relationships for enzymes embedded in the biological membrane. Working in drug discovery at AstraZeneca since 2000 in various managerial and project roles. Currently associate director biochemical assay development delivering assays for drug screening, and leading the activities around extracellular vesicles for drug delivery at AstraZeneca. Co-authored more than 70 peer-reviewed publications.

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Engineered Extracellular Vesicles for CRISPR/Cas9 Gene Editing

Friday, 26 October 2018 at 14:00

Add to Calendar ▼2018-10-26 14:00:002018-10-26 15:00:00Europe/LondonEngineered Extracellular Vesicles for CRISPR/Cas9 Gene EditingExosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The

Extracellular vesicles (EVs) represent an exciting opportunity as biological delivery vehicles for therapeutic cargo with excellent safety, low intrinsic immunogenicity, cell-specific tropism and biological delivery efficiency. There are multiple approaches for the introduction of protein and RNA cargo into EVs, including physical, chemical and cell engineering. Application of CRISPR/Cas9 requires the functional delivery of the ribonucleoprotein (RNP) complex of Cas9 enzyme and guide RNA. Engineering of the production cell line can be used to introduce cargo into the EVs, and examples for the introduction of fluorescent and luminescent proteins will be disclosed. Uptake in recipient cells can be monitored using high content imaging to provide insight in kinetics and localization. Functional delivery can be analyzed using several assays for the gene editing event (eg Cel1 and TIDE). The use of EVs for delivery of CRISPR/Cas9 has a range of exciting applications for ex vivo manipulation of primary cells and in vivo editing of genes in diseased tissue.

Add to Calendar ▼2018-10-24 00:00:002018-10-26 00:00:00Europe/LondonExosomes and Liquid Biopsies Europe 2018Exosomes and Liquid Biopsies Europe 2018 in Rotterdam, The NetherlandsRotterdam, The