| Conferences \ Organ-on-a-Chip World Congress and 3D-Bioprinting 2018 \ Organ-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A" \ Agenda \ Kara McCloskey |
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Leaf-Inspired Microvascular PatternsFriday, 5 October 2018 at 16:30  Add to Calendar ▼2018-10-05 16:30:002018-10-05 17:30:00Europe/LondonLeaf-Inspired Microvascular PatternsSELECTBIOenquiries@selectbiosciences.comThe vascularization of tissue grafts is critical for maintaining viability of the cells within a transplanted graft. A number of strategies are currently being investigated including very promising microfluidics systems. We explored the potential for generating a vasculature-patterned endothelial cells (EC) that could be integrated into distinct layers between sheets of primary cells. Bioinspired from the leaf veins, we generated a reverse mold with a fractal vascular-branching pattern that models the unique spatial arrangement over multiple length scales that precisely mimic branching vasculature. By coating the reverse mold with 50µg/ml of fibronectin and stamping enabled selective adhesion of the human umbilical vein endothelial cells (HUVECS) to the patterned adhesive matrix, we show that a vascular-branching pattern can be transferred by microcontact printing. Moreover, this pattern can be maintained transferred to a 3D hydrogel matrix and remains stable for up to 4 days. After 4 days, HUVECs can be observed migrating and sprouting into Matrigel. These printed vascular branching patterns, especially after transfer to 3D hydrogels, provide a viable alternative strategy to the prevascularization of complex tissues. |
Add to Calendar ▼2018-10-04 00:00:002018-10-05 00:00:00Europe/LondonOrgan-on-a-Chip and Body-on-a-Chip: In Vitro Systems Mimicking In Vivo Functions "Track A"SELECTBIOenquiries@selectbiosciences.com
