Sarah Heilshorn is Professor and Associate Chair in the Materials Science & Engineering Department at Stanford University. Her laboratory integrates concepts from materials science and protein engineering to design bioinspired materials for regenerative medicine, organoid culture, and bioprinting. She is a fervent supporter of diversifying the research community. She is a Fellow of the American Institute for Medical and Biological Engineering and the Royal Society of Chemistry. She serves as Associate Editor of the journal Science Advances, Special Content Editor of Acta Biomaterialia, and on the Board of Directors for the International Society for Biofabrication.
Dynamic Hydrogel Bioinks for 3D Bioprinting of Human Tissue
Tuesday, 8 November 2022 at 09:00
Add to Calendar ▼2022-11-08 09:00:002022-11-08 10:00:00Europe/LondonDynamic Hydrogel Bioinks for 3D Bioprinting of Human TissueBioprinting and Bioink Innovations for 3D-Tissues 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
3D bioprinting has emerged as a promising tool for spatially patterning cells to fabricate replicas of human tissue. To date, most bioinks have been formulated with a focus on printability, while often overlooking the cell-interactive properties of the material. Here, we present a protein-engineered bioink material designed to have viscoelastic mechanical behavior, similar to living tissue. This viscoelastic bioink is composed of an engineered protein and a recombinant polysaccharide that are crosslinked through dynamic covalent bonds, a reversible bond type that allows for cellular remodeling over time. Viscoelastic materials are challenging to use as inks, as one must tune the kinetics of the dynamic crosslinks to allow for both extrudability and long-term stability of the printed structure. We overcome this challenge through the temporary use of small molecule catalysts and competitors that modulate the crosslinking kinetics and degree of network formation. These inks are used to print a model of breast cancer cell invasion, where the inclusion of dynamic crosslinks was required to allow cell invasion. We have also demonstrated that these materials enable the growth of human intestinal organoids, human liver organoids, and human brain organoids. Altogether, we demonstrate the power of protein-engineered, dynamic bioinks to recapitulate the native cellular microenvironment towards the fabrication of human tissue.
Add to Calendar ▼2022-11-07 00:00:002022-11-08 00:00:00Europe/LondonBioprinting and Bioink Innovations for 3D-Tissues 2022Bioprinting and Bioink Innovations for 3D-Tissues 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
Add to Calendar ▼2022-11-08 09:00:002022-11-08 10:00:00Europe/LondonDynamic Hydrogel Bioinks for 3D Bioprinting of Human TissueBioprinting and Bioink Innovations for 3D-Tissues 2022 in Boston, USABoston, USASELECTBIOenquiries@selectbiosciences.com
