| Conferences \ Genomics Research Europe \ Advances in qPCR Europe \ Agenda \ Andrej-Nikolai Spiess |
| Register | Login |
Improving sigmoidal fitting of real-time PCR curvesWednesday, 5 September 2012 at 14:45  Add to Calendar ▼2012-09-05 14:45:002012-09-05 15:45:00Europe/LondonImproving sigmoidal fitting of real-time PCR curves SELECTBIOenquiries@selectbiosciences.comA major drawback of fitting sigmoidal models to real-time PCR data is the low performance in the noisy baseline region, making the extrapolation of F0 (fluorescence at cycle 0) inaccurate. We will show that modifications such as variance-weighted fitting, robust nonlinear fitting and the application of linear-quadratic-sigmoidal hybrid models can substantially increase the accuracy of deduced parameters such as F0, efficiency and threshold cycle. |
Add to Calendar ▼2012-09-04 00:00:002012-09-05 00:00:00Europe/LondonAdvances in qPCR EuropeSELECTBIOenquiries@selectbiosciences.com
