| Conferences \ Circulating Biomarkers World Congress 2015 \ Circulating DNA, Circulating RNA, Circulating Tumor Cells \ Agenda \ Tania Konry |
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Live Single Cell Functional Phenotyping in Droplet Micro-ReactorsTuesday, 24 March 2015 at 17:00  Add to Calendar ▼2015-03-24 17:00:002015-03-24 18:00:00Europe/LondonLive Single Cell Functional Phenotyping in Droplet Micro-ReactorsSELECTBIOenquiries@selectbiosciences.comCellular functional phenotype and cell-cell communication studies could make substantial progress in understanding mechanisms of immune regulation in hematologic tumors and lead to a variety of possibilities for targeted therapeutic approaches. While flow cytometry has traditionally been used to determine single fixed cell phenotypes, it cannot provide continuous measurements of proteins and secretions in the same individual live cells over time or measure the cellular phenotype during live cell-cell interaction. It is therefore different co-culture methods and immuno-assays are performed on groups of cells to study cell-cell interactions and secretions, under the assumption that all cells of a particular “type” are identical. However, recent evidence from studies of single cells reveals that this assumption is incorrect. Thus new approaches to single cell analyses and live cell-cell interaction on a single cell level are needed to uncover fundamental biological principles and ultimately improve the detection and treatment of disease. Here we propose a new droplet-based microfluidic technology to acquire live functional phenotyping of single cells of human immune system to monitor and regulate their interactions with tumor cells in hematologic cancer-relevant system. In particular, the technology proposed in this application will allow: 1) conducting dynamic and simultaneous multi-parameter analysis of both cell surface and secretions, 2) controlled delivery of regulatory agents and therapeutics to study their effect on functional phenotype of the cell 3) monitoring of cell-cell interactions on a single cell based level as well as 4) fluorescence-activated droplet sorting (FADS) system for specific cell phynotype isolation. To validate our approach live cell vaccines such as tumor specific T cells will be analyzed for their functional phenotype and interaction with tumor cells as well as sorted after for further in vivo studies. In addition, this single-cell technol |
Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonCirculating DNA, Circulating RNA, Circulating Tumor CellsSELECTBIOenquiries@selectbiosciences.com
