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SELECTBIO Conferences Circulating DNA, Circulating RNA, Circulating Tumor Cells

Tania Konry's Biography

Tania Konry, Assistant Professor, Department of Pharmaceutical Sciences, Northeastern University

Starting from training in biotechnology engineering during my doctoral studies, her interests have evolved continually to span the areas of bioengineering tools, bioassays, biosensors, microfluidics, and clinical diagnostics. Her most significant contribution in the area of microfluidics to date is the design of new sample sparing droplet-based lab on chip technologies for immunological cells capture and interrogation. Dr.Konry’s laboratory is now adopting a strong focus on technology translation to the clinic by paying attention to clinical sample handling and integration with downstream biochemical and molecular analysis on lab on a chip devices. Her focus is primarily oriented in development of new sample sparing microfluidic based assays for simultaneous multi-parameter analyses of live cells and cell-cell interactions for establishing a correlation between the immunological mechanism of the activation or inhibition of immune effectors and the targeted clinical response in disease conditions such as allergy, asthma, autoimmunity, acquired and primary immunodeficiency, transplantation, and infection contributes. In particular, Dr.Konry has extended experience in performing cutting-edge research focused on the application of biotechnology engineering toward single cell studies, and cancer immunology, material and chemical science. This expertise allow her to develop novel robust microfluidic based set-up for both generation and analysis of novel droplet based micro-sized bioreactors. These droplet-based micro-reactors will resemble immunological synapse that will allow us to mimic the therapy protocols as well as study dynamics of cell-cell interactions of immunological cells. Once the complex interactions and signaling outcomes at cell-cell interactions are defined, it should be possible to build multiple co-stimulatory signals and block the inhibitory pathways into vaccines in an appropriate combinatorial fashion.

Tania  Konry Image

Live Single Cell Functional Phenotyping in Droplet Micro-Reactors

Tuesday, 24 March 2015 at 17:00

Add to Calendar ▼2015-03-24 17:00:002015-03-24 18:00:00Europe/LondonLive Single Cell Functional Phenotyping in Droplet

Cellular functional phenotype and cell-cell communication studies could make substantial progress in understanding mechanisms of immune regulation in hematologic tumors and lead to a variety of possibilities for targeted therapeutic approaches. While flow cytometry has traditionally been used to determine single fixed cell phenotypes, it cannot provide continuous measurements of proteins and secretions in the same individual live cells over time or measure the cellular phenotype during live cell-cell interaction. It is therefore different co-culture methods and immuno-assays are performed on groups of cells to study cell-cell interactions and secretions, under the assumption that all cells of a particular “type” are identical. However, recent evidence from studies of single cells reveals that this assumption is incorrect. Thus new approaches to single cell analyses and live cell-cell interaction on a single cell level are needed to uncover fundamental biological principles and ultimately improve the detection and treatment of disease. Here we propose a new droplet-based microfluidic technology to acquire live functional phenotyping of single cells of human immune system to monitor and regulate their interactions with tumor cells in hematologic cancer-relevant system. In particular, the technology proposed in this application will allow: 1) conducting dynamic and simultaneous multi-parameter analysis of both cell surface and secretions, 2) controlled delivery of regulatory agents and therapeutics to study their effect on functional phenotype of the cell 3) monitoring of cell-cell interactions on a single cell based level as well as 4) fluorescence-activated droplet sorting (FADS) system for specific cell phynotype isolation. To validate our approach live cell vaccines such as tumor specific T cells will be analyzed for their functional phenotype and interaction with tumor cells as well as sorted after for further in vivo studies. In addition, this single-cell technol

Add to Calendar ▼2015-03-23 00:00:002015-03-24 00:00:00Europe/LondonCirculating DNA, Circulating RNA, Circulating Tumor