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SELECTBIO Conferences RNA-Seq, Single Cell Analysis & Single Molecule Analysis 2017

Roger Lasken's Biography



Roger Lasken, Director of Single Cell Genomics, J. Craig Venter Institute

Professor Lasken leads a single cell genomics program at the J. Craig Venter Institute. His group developed the MDA method (sold as TempliPhi, GenomiPhi and REPLI-g) and was the first to sequence DNA from individual cells. Current work in the lab focuses on RNA-seq from single cells and nuclei (Grindberg, R. V. et al. RNA-sequencing from single nuclei, PNAS, USA, doi:10.1073/pnas.1319700110 (2013); Krishnaswami et al., Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons, Nature Protocols (2016), vol. 11, No. 3, 499; Lacar, et al. Nuclear RNA-seq of single neurons reveals molecular signatures of activation, Nature Communications (2016) Apr 19;7:11022. doi: 10.1038/ncomms11022. PMID: 27090946.

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Capturing Transcriptomes From Single Cell Nuclei

Thursday, 5 October 2017 at 09:00

Add to Calendar ▼2017-10-05 09:00:002017-10-05 10:00:00Europe/LondonCapturing Transcriptomes From Single Cell NucleiRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

A method is presented for sequencing the transcriptome of a cell nucleus. Biological features of the nucleus are revealed including enrichment of certain transcripts and synthesis of precursors for non-coding RNAs. Current methods using protease dissociation of whole cells from tissue alters the transcriptome whereas nuclear transcriptomes are obtained without elevated temperature and harsh treatment.


Add to Calendar ▼2017-10-05 00:00:002017-10-06 00:00:00Europe/LondonRNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017RNA-Seq, Single Cell Analysis and Single Molecule Analysis 2017 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com