Computational Analysis of Human Immune RepertoireThursday, 19 April 2012 at 11:15  Add to Calendar ▼2012-04-19 11:15:002012-04-19 12:15:00Europe/LondonComputational Analysis of Human Immune Repertoire SELECTBIOenquiries@selectbiosciences.comThe immune repertoire of an individual is the collection of antigen receptors of B or T cells generated by rearrangements of V(D)J gene segments and somatic hypermutations in response to pathogens as well as autoantigens. High-throughput sequencing (HTS) enables the comprehensive examination of the repertoire which provides important information on the body’s immune state and competency in health and disease. However, new technology inevitably comes with challenges. Sequencing errors introduced during sample processing and sequencing steps can lead to misidentification of clonotypes and artificial inflation of the diversity. Moreover, sophisticated statistical and computational approaches need to be developed to evaluate the state of the immune repertoire from the large amounts of HTS data for the diagnosis and prognosis of immunological and other related diseases. I will discuss the testing experiments and computational algorithms to improve the accuracy of identifying low frequency clonotypes (i.e. somatic hypermutations), as well as the statistical approaches we developed to model the molecular evolution of the immune repertoire for disease detection and monitoring. |
Add to Calendar ▼2012-04-19 00:00:002012-04-20 00:00:00Europe/LondonNext-Gen SequencingSELECTBIOenquiries@selectbiosciences.com
