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SELECTBIO Conferences Academic Discovery Workshop

Bhavneet Bhinder's Biography



Bhavneet Bhinder, Bioinformatics Engineer, Memorial Sloan Kettering Cancer Center

Bhavneet Bhinder obtained her BSc in biotechnology from Amity Institute of Biotechnology (India), and completed her MS in bioinformatics from Georgia Institute of Technology (USA) in 2008. Since joining the HTS Core Facility in 2009, Bhavneet has been working as a computational analyst developing tools and managing large sets of screening data. She has contributed to the development of a set of tools querying RNAi screening data for off-target effects, due to miRNA, 3’UTR, or others, enabling an annotated hit nomination for experimental confirmation studies.

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Comparative Analysis of Genome-wide RNAi Screens

Tuesday, 23 September 2014 at 09:30

Add to Calendar ▼2014-09-23 09:30:002014-09-23 10:30:00Europe/LondonComparative Analysis of Genome-wide RNAi ScreensAcademic Discovery Workshop in Baltimore, MD, USABaltimore, MD, USASELECTBIOenquiries@selectbiosciences.com

We conducted genome-wide screens using an EGFP-biosensor image based reporter assay, and utilizing the two flavors of RNAi– the siRNA duplex and the shRNA hairpin technologies. The screens were performed under identical experimental conditions and method of hit nomination. A head-on comparison amongst the two screens exhibited an overlap of only 29 hits, although 15,068 genes overlapped amongst the two libraries.  I would present the results and discuss the reasons for such discrepancy in outputs.


Add to Calendar ▼2014-09-23 00:00:002014-09-25 00:00:00Europe/LondonAcademic Discovery WorkshopAcademic Discovery Workshop in Baltimore, MD, USABaltimore, MD, USASELECTBIOenquiries@selectbiosciences.com