Comparative Analysis of Genome-wide RNAi ScreensTuesday, 23 September 2014 at 09:30 Add to Calendar ▼2014-09-23 09:30:002014-09-23 10:30:00Europe/LondonComparative Analysis of Genome-wide RNAi ScreensAcademic Discovery Workshop in Baltimore, MD, USABaltimore, MD, USASELECTBIOenquiries@selectbiosciences.com We conducted genome-wide screens using an EGFP-biosensor image based reporter assay, and utilizing the two flavors of RNAi– the siRNA duplex and the shRNA hairpin technologies. The screens were performed under identical experimental conditions and method of hit nomination. A head-on comparison amongst the two screens exhibited an overlap of only 29 hits, although 15,068 genes overlapped amongst the two libraries. I would present the results and discuss the reasons for such discrepancy in outputs. |