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Genomic quantification of isolated single cells on a microfluidic device based on hot cell-direct PCRFriday, 22 May 2015 at 14:30 ![]() Gene detection and genomic quantification of isolated single cells on an original microfluidic device for the high-throughput isolation of single cells were performed. An original detection system composed of a fluorescent microscope and an automatic image acquisition was designed for the detection of PCR product of single cells. The system was applied to detect amplicon of novel PCR/RT-PCR of the genes using fluorescent probe. The genomic quantification of the isolated cells are described in this presentation. |