Conferences \ Lab-on-a-Chip & Microfluidics, Point-of-Care Diagnostics & Global Health Asia 2017 \ Agenda \ Izumi Kubo |
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Rapid Detection of Pathogenic Bacteria on a Microfluidic DiscThursday, 30 November 2017 at 15:00 Add to Calendar ▼2017-11-30 15:00:002017-11-30 16:00:00Europe/LondonRapid Detection of Pathogenic Bacteria on a Microfluidic DiscLab-on-a-Chip and Microfluidics, Point-of-Care Diagnostics and Global Health Asia 2017 in Taipei, TaiwanTaipei, TaiwanSELECTBIOenquiries@selectbiosciences.com We have developed an originally designed microfluidic disc-like device with microchannels and microchambers for single cell isolation. For the detection of genes in isolated cells on the device, hot cell-direct polymerase chain reaction (PCR) method was invented. In this study, food pathogenic bacteria such as Salmonella enterica was isolated on the disc and determined by the amplification of its specific gene based on hot cell-direct PCR using fluorescent probe. Relative fluorescence of each microchamber after/before PCR was determined to detect the bacteria. By the method, rapid and simple detection of bacterial cells was performed. |