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SELECTBIO Conferences 3D Culture Models

Jolanta Vidugiriene's Biography

Jolanta Vidugiriene, Senior Scientist, Group Leader, Promega R&D

Jolanta Vidugiriene is a Group Leader in R&D at Promega leading development of new technologies for mechanistic studies of cell survival and adaptation, particularly those involved in regulation of cellular energy metabolism.
Prior to joining Promega, Jolanta was a Principal Investigator of a Howard Hughes International Grant, where she conducted collaborative research between the Department of Biochemistry, University of Wisconsin-Madison and Vilnius University, Lithuania. Jolanta received her Ph.D. in biochemistry from Vilnius University, completed post-doctoral studies at Boston Biomedical Research Institute and Laboratory of Molecular Parasitology, Rockefeller University.

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Luminescence Approaches for Monitoring Cellular Energy Metabolism

Thursday, 9 September 2021 at 13:45

Add to Calendar ▼2021-09-09 13:45:002021-09-09 14:45:00Europe/LondonLuminescence Approaches for Monitoring Cellular Energy Metabolism3D Culture Models in Virtual Event - Eastern Daylight Time (EDT) ZoneVirtual Event - Eastern Daylight Time (EDT)

The growing trend towards understanding the role of cell metabolism in cancer, immunology, obesity, diabetes, and neurodegenerative disease has presented specific challenges in developing rapid and reliable methods for measuring the changes in metabolic pathways. Current technologies are dependent on analytical methods or enzyme couple absorbance/fluorescent assays often require laborious sample preparation and/or lack sensitivity and dynamic range for rapid and convenient metabolite detection directly in 96-well plates. Utilizing novel proluciferin substrate that in the presence of NAD(P)H is converted to luciferin and light production by luciferase, we developed a core technology for measuring key energy co-factors (NAD(P)/NAD(P)H) and extended it to multiple metabolite (glucose, lactate, glutamine, glutamate, glycerol, triglycerides) or enzyme activity assays. Here we demonstrate applicability of those assays to various formats (96-, 384- and 1536-well plates) and samples (2D, 3D cultures) using multiple automation platforms, including collecting the samples at different time points and performing multiple assays for the same sample.

Add to Calendar ▼2021-09-09 00:00:002021-09-09 00:00:00Europe/London3D Culture Models3D Culture Models in Virtual Event - Eastern Daylight Time (EDT) ZoneVirtual Event - Eastern Daylight Time (EDT)