| Conferences \ Lab-on-a-Chip and Microfluidics World Congress 2018 \ Lab-on-a-Chip and Microfluidics: Emerging Themes, Technologies and Applications "Track A" \ Agenda \ Myung-Suk Chun |
| Register | Login |
Highly Efficient Sorting of Adult Stem Cells by Optimally Designed Microfluidic Chip Filtration Coupled with Dean Flow-aided FocusingTuesday, 2 October 2018 at 15:00  Add to Calendar ▼2018-10-02 15:00:002018-10-02 16:00:00Europe/LondonHighly Efficient Sorting of Adult Stem Cells by Optimally Designed Microfluidic Chip Filtration Coupled with Dean Flow-aided FocusingSELECTBIOenquiries@selectbiosciences.comSorting out a specific human bone marrow-derived mesenchymal stem cell (hMSC) subpopulation with high self-renewal and multipotent capacities is a useful approach to enhance effectiveness of cell therapy. As the continuous sorting into three subpopulations, our group performed flow-based sorting of hMSCs by using optimally designed microfluidic chips based on the hydrodynamic filtration (HDF) principle. In this study, to further improve sorting efficiency and throughput, a spiral channel in the upper layer designed for inertial focusing by Dean flow was coupled with the lower HDF layer. In order to quantify the hMSCs’ sorting efficiency, three subpopulations were set: rapidly self-renewing (RS) (< 23 micrometer), spindle-shaped (SS), and flattened (FL) (> 35 micrometer) cells. Recovery and purity over 91% were achieved higher than those of our previous results. The results of adipogenic and osteogenic differentiations also demonstrated a successful fractionation of three subpopulations. It should be noted that RS and SS subpopulations showed higher self-renewal and multipotent capacities than those of FL one. |
Add to Calendar ▼2018-10-01 00:00:002018-10-03 00:00:00Europe/LondonLab-on-a-Chip and Microfluidics: Emerging Themes, Technologies and Applications "Track A"SELECTBIOenquiries@selectbiosciences.com
