Hannah Mamuszka's Biography

• Home
• |
• Register
• |
• Submissions
  • Submission Guidelines
  • Submissions
• |
• Agenda
  • Keynote Speakers
  • Agenda Day 1
  • Agenda Day 2
• |
• Exhibition
• |
• Venue
• |
• Media
  • Media Partners
• |
• Short Courses
  • Symposium
  • Business Courses
• |
• Posters
  • Submission Guidelines
  • Submissions
• |

Development of EXO50: Exosome Isolation and RNA Extraction Kit for Plasma

Friday, 13 September 2013 at 10:00

Add to Calendar ▼2013-09-13 10:00:002013-09-13 11:00:00Europe/LondonDevelopment of EXO50: Exosome Isolation and RNA Extraction Kit for PlasmaExosomes and Circulating Biomarkers Summit 2013 in San Diego, CASan Diego, CASELECTBIOenquiries@selectbiosciences.com

Until recently, exosome isolation and nucleic acid extraction from biofluids has required the use of ultracentrifugation, which is time consuming, inefficient, produces unreliable results, and is not relevant for use in development of a diagnostic test. With the development of the EXO50 kit for use with serum and plasma, we have demonstrated a kit that achieves comparable or better results to ultracentrifugation, but in a shorter time, and with more reliable, reproducible, robust results. In validating the EXO50 kit, we compared expression levels of specific mRNAs as well as microRNA  to ultracentrifugation and precipitation based methods. Interestingly, the kit clearly segregates microRNAs that are found on the inside of the exosome as compared to microRNAs that are Ago2-protein bound something that ultracentrifugation and precipitation methods often do not allow for. The EXO50 kit is scalable and linear, requiring as little as 200uL and as much as 4mL in the current version, allowing for competent analysis of a single mutation or biomarker discovery using array or sequencing of thousands of genes. The fast, easy, and robust protocol of the EXO50 kit will allow for plasma based IVD development.