Targeted Integration and Expression of shRNA using Zinc Finger NucleasesTuesday, 4 September 2012 at 12:15  Add to Calendar ▼2012-09-04 12:15:002012-09-04 13:15:00Europe/LondonTargeted Integration and Expression of shRNA using Zinc Finger NucleasesSELECTBIOenquiries@selectbiosciences.comViral delivery of shRNA has been a powerful tool to investigate gene function in various cellular systems. The integration of virally delivered constructs occurs randomly within the genome, which can disrupt non-targeted genes, potentially causing undesirable phenotypes and causing epigenetic cell-to-cell variations. Now, using targeted genome editing technology, we can deliver an shRNA expressing construct directly to a single endogenous “safe harbor” locus within the human genome. This approach enables shRNA expression without disrupting surrounding genes, leading to an efficient knockdown of the target gene. Here, we will discuss studies demonstrating the combination of genome-wide shRNA library coverage with targeted integration using Zinc Finger Nucleases. |
Add to Calendar ▼2012-09-04 00:00:002012-09-05 00:00:00Europe/LondonRNAi and miRNASELECTBIOenquiries@selectbiosciences.com
