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SELECTBIO Conferences Extracellular Vesicle-based Dx & Rx Summit

Aurelio Lorico's Biography

Aurelio Lorico, Professor of Pathology, Touro University Nevada

Aurelio Lorico did his post-doc at Yale University, mentored in the pharmacology field by Alan Sartorelli. Later, as Junior Research Faculty at Yale he clarified the biological function(s) of the MRP1 gene by generating and characterizing MRP1 knockout mice and cell lines. After 8 years at Yale, he became senior scientist at the Norwegian Cancer Center in Oslo, working on cancer drug resistance and designing new strategies for gene therapy of hereditary diseases. Back to the US, he has been working on cell-to-cell communication in the tumor microenvironment, particularly cell-cell fusion and extracellular vesicles, and on the development of innovative therapeutic strategies for breast cancer, melanoma and glioblastoma. His lab has recently discovered spathasomes, organelles constituted by Rab7+ late endosomes entering into nuclear envelope invaginations that mediate the nuclear translocation of EV-derived biomaterials, such as proteins and RNA.

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Extracellular Vesicles Go Nuclear

Thursday, 28 March 2019 at 17:30

Add to Calendar ▼2019-03-28 17:30:002019-03-28 18:30:00Europe/LondonExtracellular Vesicles Go NuclearExtracellular Vesicle-based Dx and Rx Summit in Coronado Island, CaliforniaCoronado Island,

Molecular mechanisms regulating EV biogenesis, their release, and subsequent uptake by target cells have emerged during the last two decades. How their cargo molecules are selectively delivered to their intracellular sites of action, including the intra-nuclear compartment, is still obscure. This issue is particularly important given that the biogenesis and functionality of EVs are dysregulated under pathological conditions. Recently, we described a novel sub-nuclear compartment which is created by the entry of small GTPase Rab7-containing late endosomes in the nucleoplasmic reticulum. The latter is shaped by superficial and deep nuclear envelope invaginations (NEI) penetrating into the nucleoplasm. Given that late endosomes in NEI has often an elongated appearance and resembles a sword in its scabbard, we proposed to name this dual-structure “spathasome” from Greek/Latin words “spathi/spatha” for sword. This structure appears to act as an intermediate compartment for the delivery of the content of endocytosed EVs (e.g., CD9/CD133 protein complexes and RNA molecules) to the nucleoplasm of their host cell. The NEI-associated late endosomes and nuclear localization of EV-derived proteins were observed in cancer cells and mesenchymal stromal cells in cultures and in breast cancer patient biopsies. A molecular complex, investigated by indirect immunofluorescence, fluorescence resonance energy transfer, immunoisolation techniques and RNA interference, was found to be responsible for the entry of EV cargo into the nucleoplasmic reticulum.

Add to Calendar ▼2019-03-28 00:00:002019-03-28 00:00:00Europe/LondonExtracellular Vesicle-based Dx and Rx SummitExtracellular Vesicle-based Dx and Rx Summit in Coronado Island, CaliforniaCoronado Island,