Held in conjunction with 3D-Culture, Organoids & Tox Screening Europe 2019
13 Jun 2019, at 19:00-21:30 in Rotterdam, The Netherlands
Price:
Introduction to the Training Course
3D
cell culture models present a challenge for using imaging methods and
assay chemistries that were originally designed for measuring events
from 2D monolayers of cells. The size of 3D structures and the added
complexity of scaffolds and “chip devices” used for culturing often
present barriers to directly measuring markers to interrogate biological
function.
This workshop will present basic strategies and
factors to consider for designing and validating assays applied to 3D
culture models. We will discuss case studies describing reagent design
efforts and alternate approaches to overcome the limitations inherent
with detecting markers from 3D culture models.
Topics Covered in this Training Course
- Introduction to 3D-Culture
- Why 3D-Culture and Its Utility in Basic Research and the Pharmaceutical Industry
- Challenges in the Deployment of 3D Culture Protocols
- Designing
detection reagents and appropriate controls to confirm lysis of all
cells in 3D culture model systems measuring markers released into
culture medium
- The use and penetration of small molecule probes to and interrogate biology within 3D structures
- The potential to apply real time multiplex detection methods with 3D models for high throughput screening
Who Should Attend
- Basic Researchers Seeking to Bring 3D-Culture into their Research Environment
- Pharmaceutical Industry Researchers
- Technology and Tools Providers Seeking an Understanding of the Opportunities in this Field and Potential for Product Development
This
is an interactive dinner training course-a light dinner and
refreshments are served to all course attendees. We plan to include
time for informal open and interactive discussion of topics and benefit
from sharing of experiences among participants. All
course participants will receive the slide decks used for the workshop
and also latest reviews and publications of value for R&D
presentations, business plans and investor presentations.
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Terry Riss, Global Strategic Marketing Manager Cell Health, Promega Corporation
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