Tumor-derived Extracellular Vesicles Require ß1 Integrins to Promote Anchorage-Independent Growth

Friday, 29 March 2019 at 11:00

Add to Calendar ▼2019-03-29 11:00:002019-03-29 12:00:00Europe/LondonTumor-derived Extracellular Vesicles Require ß1 Integrins to Promote Anchorage-Independent GrowthCirculating Biomarkers World Congress 2019 in Coronado Island, CaliforniaCoronado Island, CaliforniaSELECTBIOenquiries@selectbiosciences.com

The ß1 integrins, known to promote cancer progression, are abundant in extracellular vesicles (EVs).  We investigated whether prostate cancer (PrCa) EVs affect anchorage-independent growth and whether ß1 integrins are required for this effect.  Specifically using a cell line-based genetic rescue and an in vivo PrCa model, we show that gradient purified small EVs (sEVs) from either cancer cells or blood from tumor-bearing TRAMP mice promote anchorage-independent growth of PrCa cells.  In contrast, sEVs from cultured PrCa cells harboring a shRNA to ß1, from wild-type mice or from TRAMP mice carrying a ß1 conditional ablation in the prostatic epithelium (ß1pc-/-), do not.  We find that sEVs, from cancer cells or TRAMP blood, are functional and co-express ß1 and sEV markers; in contrast, sEVs from ß1pc-/- /TRAMP or wild-type mice lack ß1 and sEV markers.  Our results demonstrate that ß1 integrins in tumor-derived sEVs are required for stimulation of anchorage-independent growth.