Jack Keene,
James B. Duke Professor
Education: University of California, Riverside 1969; PhD in Microbiology and Immunology, University of Washington, Seattle 1974. I trained in biochemistry and virology as a fellow at the NIH 1974-1978 where I learned classical Sanger sequencing and Maxim-Gilbert rapid sequencing of virus genomes. Joined the faculty Duke University Medical Center in 1979 as Assistant Professor of Microbiology and Immunology, with interest in virus-host interactions, virus genomes, and regulatory mechanisms of RNA biology. Professor and Chairman of Microbiology (1992-2002). James B. Duke Distinguished Professor 1994-Present.
Highlights of research: My lab was first to clone a sequence specific RNA-binding protein, La (1985), and thereby discovered and named the RNA Recognition Motif (RRM) and RRM family proteins (1988-1989). We devised novel methods to study mechanisms of protein-RNA interactions (1984-90), discovered low complexity repeats in U1- 70k RBP (1989) that caused liquid-coalesced in the nucleus, cloned ELAVL2 (HuB) RBP (1990), and showed that low complexity repeats inhibited splicing and nuclear export (1994-95). We were first to demonstrate multi-targeting of a RNA binding proteins (1993-94), and to that end we invented the RIP and CLIP methods (1999) that allowed us to discover RNA regulons (2000-present). We then demonstrated quantitative changes in RBP binding site strength across the entire transcriptome (2010-2017), and demonstrated RAS protein activates post-transcriptional events leading to malignant invasive phenotype (2018).
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