Poster Presentations
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Liquid biopsy for peritoneal metastasis of gastric cancer using exosomal microRNA in peritoneal fluid.
Hideyuki Ohzawa, Center for Clinical Research, Jichi Medical University
Objectives: Peritoneal metastasis (PM) frequently occurs in patients with gastric cancer and has poor prognosis. However, there is no reliable biomarker associated with peritoneal metastasis. Exosomal miRNA profiles in peritoneal fluids from patients with gastric cancer were examined and biomarkers associated with PM were explored.
Methods: Peritoneal fluids were obtained either laparoscopically, during open surgery or from subcutaneous infusion port connected to an intraperitoneal catheter. Exosome fractions were isolated using ultracentrifuge method. Total RNA including small RNA was extracted from exosome fractions and expression of miRNAs were analyzed using RT-PCR.
Result: At first, expression of miRNAs were comprehensively analyzed using samples from patients with PM and without PM. Eleven miRNAs were showed differential expression between the 2 groups. Several miRNAs were selected and further analyzed using peritoneal lavage fluids collected from patients received curative gastrectomy. Low expression of miR-29b-3p were significantly associated with peritoneal recurrence.
Conclusion: Several dysregulated exosomal miRNAs associated with PM from gastric cancer were identified in this study. Exosomal miRNA profiles in peritoneal fluids might be useful biomarkers to predict the peritoneal recurrence.
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