Poster Presentations
|
Circulating miRNAs as Early Diagnostic Biomarkers of Breast Cancer
Katarina Cuk, PhD student, DKFZ
In recent years circulating miRNAs have attracted a great deal of attention as promising novel biomarkers for various diseases. The aim of this study was to investigate their potential to serve as minimally invasive, early detection biomarkers for breast cancer in blood plasma. In an initial screening step plasma of early stage breast cancer patients and healthy individuals were profiled by TaqMan® Low Density Arrays. Candidate miRNAs were further validated in an extended study cohort of 207 individuals including 127 breast cancer cases and 80 healthy controls via RT-qPCR. Four miRNAs (miR-A, miR-B, miR-C and miR-D) were shown to be significantly upregulated in the plasma of breast cancer patients. Their diagnostic potential was evaluated by ROC curve analysis. For individual miRNAs AUCs ranged between 0.69-0.71. Since miR-A and miR-B expressions correlated highly, the most informative and least redundant diagnostic panel comprised only three miRNAs: miR-A, miR-C and miR-D (AUC=0.76). In conclusion, this panel of circulating miRNAs might have the potential to complement and improve early diagnosis of breast cancer.
|
|
