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The study of the microfluidic biosensor chip for nucleic acid rapid detection and its applications
Yunrui Han, Research associate, Institute of Marine Science and Technology, Shandong University
This thesis takes advantages of the interdisciplinary of microelectronics technology, biology and chemistry, and is based on microelectronics technology, new nanomaterials and biomolecular materials. A novel microfluidic biosensor chip for the detection of (mi)RNA biomarkers was constructed by using local self-assembly technology and micro-nano fabrication process. The microfluidic biosensor chips was obtained with high throughput, wide dynamic detection range and ultra-low detection limit by designing and optimizing the parameters of the chip structure and the detection process. The excellent clinical detection capability of the microfluidic biosensor chips was proved by the detection of (mi)RNA biomarkers in clinical samples.
Microfluidic model of 3D tumor spheroids for drug screening
Yihe Wang, Associate research scientist, Shandong University
The dearth of preclinical models that accurately represent the dynamic nature of tumor microenvironments is one of the challenges impeding progress in the creation of effective cancer medicines. Despite the fact that interstitial flow has a significant influence on tumor response to chemotherapy, traditional in vitro cancer models typically ignore this important aspect. This article reports on a proof-of-concept microfluidic technology for the production of massive arrays of breast tumor spheroids that are grown in a biomimetic hydrogel at flow conditions that are nearly physiological. For research of the effects of medication dose and supply rate on the chemosensitivity of breast tumor spheroids, this cancer spheroids-on-a-chip model is utilized. Large arrays of tumor spheroids may be grown using patient-derived cells from various breast cancer subtypes, and a correlation between in vivo medication effectiveness and on-chip spheroid drug responsiveness is observed. The suggested platform can be used to effectively screen new anticancer drugs and develop personalized cancer therapy in vitro.
Convenient Fabrication of Flexible, Transparent Teflon-FEP (Fluorinated Ethylene Propylene) Microfluidic Film Chips
Zhenghao WANG, PhD Candidate, Hong Kong University of Science and Technology
This study presents a novel, simple, and cost-effective method for fabricating Teflon FEP microfluidic film chips. Micropatterns were generated on a thin FEP film (~50 µm) by micro-embossing and sealed with another flat FEP thin film (~300 µm) to form microchannels. Our approach enables rapid prototyping of lab-on-a-film FEP microchips at low cost and with minimal machine requirements. We investigated the cross-sectional profile of microchannels to enhance the effectiveness and versatility of our method. In addition to the inherent benefits of Teflon FEP, the thin film of microchannels allows for the integration of beneficial functionalities, including efficient heat transfer and mechanical flexibility. Moreover, a glue-free connector was designed to interface the thin-film microdevices with the macroscopic world, improving the stability of fluid delivery inside the film-based FEP microchips. The connector remains leak-tight without glue, making the platforms compatible with organic solvents. Our developed FEP microfluidic chips enrich the microfluidics toolbox with powerful and versatile features and functionalities. We believe that the FEP lab-on-a-film platform will become an attractive option for a wide range of microfluidic applications, including biosensing and organic reactions.
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