Poster Presentations
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Temperature control and stabilization system for microfluidic cartridge in EIS biosensor application.
Pawel Polanski, Embedded Engineer, R&D Team Leader, SensDx S.A.
One of the major issues to overcome during the development of microfluidic systems is sample temperature conditioning. For the project evaluation a microfluidic cartridge was designed in order to provide a thermal separation between electrochemical impedance measurement zone and sample amplification chamber. This separation is absolutely crucial because the amplification process requires a constant temperature of 55 Celsius degree where EIS electrodes are covered with biofilm that will start to degrade in such a temperature. Target of a project is to create an easy to use, affordable test. Therefore no expensive materials and equipment were available to be utilized. Cartridge designed in a CAD program and then 3D printed using Polypropylene due to its thermal resistance. Design assumptions provide temperature measurement from two points: one in the thermal block which delivers heat to the target and another one at the top of the reaction chamber. In order to stabilize the requested temperature model was simulated using FFEPlus iterative solver. Results were confirmed with IR imaging and actual reading in a laboratory environment. Summing up, it is possible to create a low-cost indirect multipoint temperature measurement system in microfluidic application.
Optimizing the functionalization of super-paramagnetic beads for droplet-based protein secretion profiling of immune cells
Lucas Santin, , IRT Bioaster
The extremely dynamic functions of an active immune response are mainly determined by the presence of various soluble factors. These secreted factors organize the individual immune cells into functional tissues, regulating the immune response, making single-cell studies fundamental to investigate immune cells’ behaviors heterogeneity[1]. The powerful improvement provided by droplet-based microfluidics deciphering the dynamic processes at the single-cell level could help to describe the fundamental mechanisms underlying immunity[2], to develop new strategies for vaccination, cancer immunotherapy and to diagnose inflammatory diseases. Recently, our group proposed the DropMap system to study a range of different immune cells, analyzing the secretion of a variety of different proteins, and variety of murine and human cytokines[3].
An important key feature of our droplet-based immunoassay is the use of functionalized paramagnetic nanoparticles. This work describes the optimizations of various functionalization methods for super-paramagnetic nanobeads integrated in droplet-based microfluidics. Particularly, three methods using different croslinkers were considered to give an analysis of which method is useful to obtain an improved and reproducible fluorescent signal suitable for single-cell analysis. In addition, as applicative experiment, the encapsulation of single immune cells was performed, to investigate the potential of the functionalized beads for sensible and early detection of protein secretion.
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