Poster Presentations
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Microdevice immunoassay with conjugated magnetic nanoparticles for rapid anti-cyclic citrullinated peptide (anti-CCP) detection
Kenia Chávez Ramos, PhD student, Universidad Nacional Autónoma de México
Anti-cyclic citrullinated peptide IgG antibodies (anti-CCP) are produced as an immune response in the presence of post-translational modified peptides known as cyclic citrullinated peptides (CCP). These autoantibodies have been considered as specific biomarkers for the diagnosis of rheumatoid arthritis (RA). The most widely used method for anti-CCP detection is an enzyme-linked immunosorbent assay (ELISA). Despite its great sensitivity, ELISA is considered a time-consuming assay. For this paper, we used a simple straight line microdevice and CCP conjugated magnetic nanoparticles (MNPs-CCP) as solid support for quantifying anti-CCP. The spectrophotometric detection was carried out by using a microdevice with an optical flow Z cell design coupled with optical fibers. The microdevice immunoassay, employing only 6 microliters of sample and reagents, is shown in this paper to be almost nine times faster than a commercial anti-CCP ELISA kit but with equivalent results. The detection range was 0.70-2000 units per milliliter with a limit of detection of 0.70 units per milliliter (16 times more sensitive than the compared ELISA kit). The microdevice immunoassay, with conjugated MNPs-CCP, offers a cheaper, faster, more sensitive and simple method for anti-CCP quantification.
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