Poster Presentations
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A high-throughput 3-dimensional cartilage tissue engineering platform using fetal cartilage-derived progenitor cells (FCPCs) and a scaffold-free tissue fabrication method
Bo Ram Song, , Inha University/ Global Stem cell & Regenerative medicine Acceleration Center
Stem cells are a valuable tool in drug development. However, their application has still limitations because it needs complicated and multistep differentiation process, which might not be efficient enough to represent specific cellular or disease phenotypes nor adoptable to high-throughput drug screening and testing. In this study, we developed a proprietary and simple technology to construct a highly mature artificial cartilage using fetal cartilage-derived progenitor cells (FCPCs) and a scaffold-free tissue fabrication method. Human FCPCs were isolated from GA 12-16 fetus at Ajou University Hospital with the IRB approval (AJIRB-CRO-07-139) and the written consent of the donor. We have previously reported human FCPCs are highly proliferative and potent in cartilage tissue formation, thereby could be good cell source for cartilage regeneration and related applications1. The scaffold-free cartilage fabrication method involves high-density cell culture sequentially in 2D and 3D environment. When FCPCs were combined with the fabrication method, they produced a highly mature artificial cartilage tissues that shows native cartilage phenotypes in the histological and biochemical properties. We adopted multi-channel plates and nano- or micro-patterned plates to produce a high-throughput artificial cartilage fabrication platform for drug development use.
A high-throughput 3-dimensional cartilage tissue engineering platform using fetal cartilage-derived progenitor cells (FCPCs) and a scaffold-free tissue fabrication method
Bo Ram Song, , Inha University/ Global Stem cell & Regenerative medicine Acceleration Center
Stem cells are a valuable tool in drug development. However, their application has still limitations because it needs complicated and multistep differentiation process, which might not be efficient enough to represent specific cellular or disease phenotypes nor adoptable to high-throughput drug screening and testing. In this study, we developed a proprietary and simple technology to construct a highly mature artificial cartilage using fetal cartilage-derived progenitor cells (FCPCs) and a scaffold-free tissue fabrication method. Human FCPCs were isolated from GA 12-16 fetus at Ajou University Hospital with the IRB approval (AJIRB-CRO-07-139) and the written consent of the donor. We have previously reported human FCPCs are highly proliferative and potent in cartilage tissue formation, thereby could be good cell source for cartilage regeneration and related applications1. The scaffold-free cartilage fabrication method involves high-density cell culture sequentially in 2D and 3D environment. When FCPCs were combined with the fabrication method, they produced a highly mature artificial cartilage tissues that shows native cartilage phenotypes in the histological and biochemical properties. To our surprise, the cartilage tissue had a paste-like physical property and could be fabricated in a variable diameter. We adopted multi-channel plates and nano- or micro-patterned plates to produce a high-throughput artificial cartilage fabrication platform for drug development use.
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