Poster Presentations
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Mobile Diagnostic System for the Point-of-Care-Detection of Infectious Diseases by Integrated Electrical Biochip Cartridges
Lars Blohm, Research Scientist, Fraunhofer Institute for Silicon Technology
Mobile diagnostics provide higher quality of life to the patients since the point-of-care diagnostics implies that physicians or even trained patients are able to execute analyzing tests on their own. Therefore mobile analysis systems have to provide easy to handle and rapid tests with comparable sensitivities to the systems of the central laboratories.
The Fraunhofer ISIT develops a silicon based biochip platform which is applicable for a wide range of immunological based tests (ELISA). Therewith the patient is able to run a multi-parametric analysis of e.g. HCV antibodies out of only two microliters whole blood within seven minutes. The system comprises a disposable cartridge equipped with required reagents, fluidic structures and integrated biochip. Next to easy sample collection and automated sample dilution the microfluidic immunological assay on gold microelectrodes takes place. Furthermore the combination of temperature control and “single electrode redox cycling” for amperometric signal amplification during electrochemical detection are important methods. The mobile system has only electrical and mechanical interfaces to the disposable cartridge modules and provides simultaneous measuring of a reference sample for calibration purpose.
Overall the biochip platform combines micro system technology and modern methods of molecular biology resulting in multi-parametric point-of-care tests.
A Novel Microfluidic Device for Cancer Screening and Single Cell Drug Response Study
Huseyin Kizil, Associate Professor, Istanbul Technical University
We present a microfluidic based platform to capture target type of cells as single cell array and perform impedance analysis for further analysis. Introducing a lab-on-a-chip device with two different active regions to define single cell physical/electrical characteristics for diagnosing a disease and proposing a potential drug is our primary research goal. First active region: dielectrophoresis is used for cell separation and enrichment. Second active region: Impedance analysis is adopted for cell detection, classification and characterization. Titanium planar interdigitated microelectrodes are placed with an angle of 45° to perform dielectrophoresis based cell separation and the cells are continuously captured through a novel three dimensional PDMS microchannel as single cell for each trapping regions. An interdigitated microelectrode couple is placed under each trapping area to record the impedance shift caused by cell entrapment. Impedance shift varies with the difference of cell type, medium conductivity and applied frequency. This technique will be used for calculating the physical/electrical properties of interior cell structures and the separation efficiency by DEP will be increased with gained knowledge of target cell lines. Chemical stimulation on a target cell using a specific type of drug could also be evidenced by the amount of these impedance shifts.
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